PMID- 18641179 OWN - NLM STAT- MEDLINE DCOM- 20090121 LR - 20081127 IS - 1525-3163 (Electronic) IS - 0021-8812 (Linking) VI - 86 IP - 12 DP - 2008 Dec TI - Differential expression in lung and bronchial lymph node of pigs with high and low responses to infection with porcine reproductive and respiratory syndrome virus. PG - 3279-89 LID - 10.2527/jas.2007-0685 [doi] AB - One hundred Hampshire x Duroc cross-bred pigs and 100 Nebraska Index line pigs were infected with porcine reproductive and respiratory syndrome virus (PRRSV) and evaluated for resistance and susceptibility. Controls (100/line) were uninfected littermates to infected pigs. Viremia (V), BW change (WTDelta), and rectal temperature at 0, 4, 7, and 14 d postinfection were recorded. Lung, bronchial lymph node (BLN), and blood tissue were collected at necropsy (14 d postinfection). Infected pigs were classified as low or high responders to PRRSV based on the first principal component from principal component analyses of all variables. Low responders to PRRSV (low PRRSV burden) and their uninfected littermates were assigned to the low (L) class. High responders to PRRSV (high PRRSV burden) and their uninfected littermates were assigned to the high (H) class. Infected pigs in the L class had large WTDelta, low V, and few lung lesions; H-class pigs had small WTDelta, high V, and many lung lesions. Ribonucleic acid was extracted from lung and BLN tissue of the 7 highest and 7 lowest responders per line and from each of their control littermates. A control reference design was used, and cDNA from each reference sample tissue was prepared from pooled RNA extracted from 2 control pigs from each line whose infected littermates had a principal component value of 0. Design variables in data analyses were line (Index vs. Hampshire x Duroc), class (H vs. L), treatment (infected vs. uninfected controls), and slide/pig as error. Oligo differential expression was based on P < 0.01 occurring in both lung and BLN. Line and treatment effects were significant for 38 and 541 oligos, respectively, in both lung and BLN. Line x class interaction existed for expression of thymosin beta-4, DEAD box RNA helicase 3, acetyl-cholinesterase, and Homo sapiens X (inactive)-specific transcript in both tissues. Treatment x class existed for expression of CCAAT/enhancer-binding delta protein, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha, thioredoxin-interacting protein, major facilitator superfamily domain containing 1, and unknown sequences SS00012040 and SS00012343. Line x treatment and line x treatment x class interactions were not significant. Possible important genetic associations for fine-mapping candidate genes related to response to PRRSV and determining causative alleles were revealed. FAU - Bates, J S AU - Bates JS AD - Department of Animal Science, University of Nebraska, Lincoln 68583-0908, USA. FAU - Petry, D B AU - Petry DB FAU - Eudy, J AU - Eudy J FAU - Bough, L AU - Bough L FAU - Johnson, R K AU - Johnson RK LA - eng GR - P20 RR016469/RR/NCRR NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20080718 PL - United States TA - J Anim Sci JT - Journal of animal science JID - 8003002 SB - IM MH - Animals MH - Bronchi/metabolism MH - Female MH - *Gene Expression Regulation MH - Immunity, Innate/genetics MH - Lung/*metabolism MH - Lymph Nodes/*metabolism MH - Male MH - Porcine Reproductive and Respiratory Syndrome/*genetics/*metabolism MH - Porcine respiratory and reproductive syndrome virus/physiology MH - Swine/*metabolism/*virology EDAT- 2008/07/22 09:00 MHDA- 2009/01/22 09:00 CRDT- 2008/07/22 09:00 PHST- 2008/07/22 09:00 [pubmed] PHST- 2009/01/22 09:00 [medline] PHST- 2008/07/22 09:00 [entrez] AID - jas.2007-0685 [pii] AID - 10.2527/jas.2007-0685 [doi] PST - ppublish SO - J Anim Sci. 2008 Dec;86(12):3279-89. doi: 10.2527/jas.2007-0685. Epub 2008 Jul 18.