PMID- 18642604
OWN - NLM
STAT- MEDLINE
DCOM- 20080922
LR  - 20190902
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 410
DP  - 2008
TI  - Laboratory methods for the detection of chromosomal structural aberrations in 
      human and mouse sperm by fluorescence in situ hybridization.
PG  - 241-71
AB  - The father, like the mother, can transmit genetic defects that are detrimental 
      for development and genetic health for his children, but the mechanisms for 
      paternally mediated abnormal reproductive outcomes remain poorly understood. A 
      battery of sensitive methods has been developed for detecting genetic damage 
      associated with infertility, spontaneous abortions, as well as inherited defects 
      in children such as aneuploidy syndromes, translocation carriers, and certain 
      genetic diseases directly in sperm. Among these, fluorescence in situ 
      hybridization (FISH) sperm-based assays for measuring numerical abnormalities and 
      structural chromosomal aberrations are now available for an expanding number of 
      species including humans, rodents, and several domesticated animals. This new 
      generation of sperm FISH methods has identified several paternal risk factors 
      such as age, various drugs, lifestyles, and various environmental and 
      occupational exposures. These sperm FISH assays provide new opportunities to 
      identify and characterize male reproductive risks associated with genetic, 
      lifestyle, and environmental factors. This chapter outlines the laboratory 
      methods for the detection of sperm with chromosomal structural aberrations in 
      humans (ACM assay) and mice (CT8 assay) that have been validated for detecting 
      environmental germ cell mutagens.
FAU - Marchetti, Francesco
AU  - Marchetti F
AD  - Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
FAU - Cabreros, Debby
AU  - Cabreros D
FAU - Wyrobek, Andrew J
AU  - Wyrobek AJ
LA  - eng
GR  - Y01-ES-8016-5/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Hazardous Substances)
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Environmental Monitoring/methods
MH  - Hazardous Substances/toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Mice
MH  - Reproducibility of Results
MH  - Spermatozoa/drug effects/*metabolism
EDAT- 2008/07/23 09:00
MHDA- 2008/09/23 09:00
CRDT- 2008/07/23 09:00
PHST- 2008/07/23 09:00 [pubmed]
PHST- 2008/09/23 09:00 [medline]
PHST- 2008/07/23 09:00 [entrez]
AID - 10.1007/978-1-59745-548-0_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;410:241-71. doi: 10.1007/978-1-59745-548-0_14.