PMID- 18644019
OWN - NLM
STAT- MEDLINE
DCOM- 20081231
LR  - 20081006
IS  - 1365-2133 (Electronic)
IS  - 0007-0963 (Linking)
VI  - 159
IP  - 4
DP  - 2008 Sep
TI  - Abnormal DNA methylation in T cells from patients with subacute cutaneous lupus 
      erythematosus.
PG  - 827-33
LID - 10.1111/j.1365-2133.2008.08758.x [doi]
AB  - BACKGROUND: Impaired methylation of T-cell DNA is thought to contribute to the 
      development of systemic lupus erythematosus. However, it is unknown whether 
      T-cell hypomethylation is a factor in other, less severe, forms of lupus 
      erythematosus such as subacute cutaneous lupus erythematosus (SCLE). OBJECTIVES: 
      To investigate global DNA methylation and the expression of genes that regulate 
      methylation in T cells of patients with SCLE. METHODS: We quantified global 
      methylcytosine levels in CD4+ and CD8+ T cells from 12 patients with SCLE and 
      nine healthy controls. mRNA levels of DNA methyltransferases (DNMTs), methylated 
      CpG binding proteins (MBDs) and CD11a were measured by real-time quantitative 
      polymerase chain reaction. RESULTS: CD4+ T-cell DNA from patients with SCLE was 
      hypomethylated relative to controls (P = 0.002). DNMT1 and DNMT3a mRNA levels 
      were significantly lower in CD4+ T cells from SCLE patients than in controls (P = 
      0.027 and P = 0.004, respectively). Relative to controls, MBD1, MBD3 and MBD4 
      mRNA levels were significantly higher in SCLE CD4+ cells (P < 0.001, P < 0.001 
      and P = 0.001, respectively), whereas MECP2 and MBD4 mRNA expression were 
      significantly increased in SCLE CD8+ T cells (P = 0.001 and P = 0.001, 
      respectively). DNMT1 expression positively correlated with CD4+ T-cell DNA 
      methylation within our SCLE patient cohort (r = 0.590, P = 0.044). CD11a mRNA 
      expression was significantly increased in SCLE CD4+ T cells relative to controls 
      (P = 0.044) and negatively correlated with DNA methylation (r = -0.669, P = 
      0.049). CONCLUSIONS: These data suggest that aberrant regulation of DNA 
      methylation in CD4+ T cells is associated with the development of SCLE.
FAU - Luo, Y
AU  - Luo Y
AD  - Department of Dermatology, Epigenetic Research Centre, Second Xiangya Hospital, 
      Central South University, Changsha, China.
FAU - Li, Y
AU  - Li Y
FAU - Su, Y
AU  - Su Y
FAU - Yin, H
AU  - Yin H
FAU - Hu, N
AU  - Hu N
FAU - Wang, S
AU  - Wang S
FAU - Lu, Q
AU  - Lu Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080717
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - EC 2.1.1.- (DNA Modification Methylases)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - *DNA Methylation
MH  - DNA Modification Methylases/*genetics
MH  - Female
MH  - Gene Expression/genetics
MH  - Humans
MH  - Lupus Erythematosus, Cutaneous/*genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes/immunology/*metabolism
EDAT- 2008/07/23 09:00
MHDA- 2009/01/01 09:00
CRDT- 2008/07/23 09:00
PHST- 2008/07/23 09:00 [pubmed]
PHST- 2009/01/01 09:00 [medline]
PHST- 2008/07/23 09:00 [entrez]
AID - BJD8758 [pii]
AID - 10.1111/j.1365-2133.2008.08758.x [doi]
PST - ppublish
SO  - Br J Dermatol. 2008 Sep;159(4):827-33. doi: 10.1111/j.1365-2133.2008.08758.x. 
      Epub 2008 Jul 17.