PMID- 18647361
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20211020
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 72
IP  - 4
DP  - 2008 Oct
TI  - A Taqman assay for high-throughput genotyping of the multiple 
      sclerosis-associated HLA-DRB1*1501 allele.
PG  - 401-3
LID - 10.1111/j.1399-0039.2008.01101.x [doi]
AB  - The human leukocyte antigen (HLA)-DRB1*1501 allele has long been established as 
      the main genetic risk factor for multiple sclerosis (MS), and it therefore 
      follows that stratification of study populations for this allele could aid in the 
      identification of novel susceptibility genes and/or in establishing interactions. 
      To this end, we have developed a simple Taqman-based assay allowing 
      cost-efficient medium-throughput HLA-DRB1*1501 genotyping. We have validated this 
      assay in 444 trio families with MS and 1066 individuals from the UK 1958 birth 
      cohort (3908 independent chromosomes). In this validation cohort, the correlation 
      coefficient (r(2)) between rs3135388*A and HLA-DRB1*1501 was >0.94. Subsequently, 
      applying the assay to a group of MS patients and controls from Belgium confirmed 
      the association of HLA-DRB1*1501 and MS in this population (P = 5 x 10(-21)).
FAU - Goris, A
AU  - Goris A
AD  - Laboratory of Neuroimmunology, Section of Experimental Neurology, Katholieke 
      Universiteit Leuven, Leuven, Belgium. an.goris@med.kuleuven.be
FAU - Walton, A
AU  - Walton A
FAU - Ban, M
AU  - Ban M
FAU - Dubois, B
AU  - Dubois B
FAU - Compston, A
AU  - Compston A
FAU - Sawcer, S
AU  - Sawcer S
LA  - eng
GR  - 730/MSS_/Multiple Sclerosis Society/United Kingdom
PT  - Journal Article
PT  - Validation Study
DEP - 20080717
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*15:01 antigen)
SB  - IM
MH  - Alleles
MH  - Cohort Studies
MH  - Gene Frequency/*genetics
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Multiple Sclerosis/*genetics
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA/*methods
EDAT- 2008/07/24 09:00
MHDA- 2009/05/19 09:00
CRDT- 2008/07/24 09:00
PHST- 2008/07/24 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
PHST- 2008/07/24 09:00 [entrez]
AID - TAN1101 [pii]
AID - 10.1111/j.1399-0039.2008.01101.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2008 Oct;72(4):401-3. doi: 10.1111/j.1399-0039.2008.01101.x. 
      Epub 2008 Jul 17.