PMID- 18647857
OWN - NLM
STAT- MEDLINE
DCOM- 20090903
LR  - 20090716
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 68
IP  - 8
DP  - 2009 Aug
TI  - Regulatory IL4+CD8+ T cells in patients with ankylosing spondylitis and healthy 
      controls.
PG  - 1345-51
LID - 10.1136/ard.2008.088120 [doi]
AB  - OBJECTIVES: Interleukin (IL)4+CD8+ regulatory T cells (Treg) obtained from 
      peripheral blood mononuclear cells (PBMCs) from patients with ankylosing 
      spondylitis (AS) by coculture with autologous dendritic cells (DC) have been 
      previously described. In the present work, the proportions of IL4+CD8+ T cells in 
      PB from patients with AS and controls are examined; in addition the conditions 
      required for the generation of IL4+CD8+ Treg cells and their frequency in T cell 
      lines from patients with AS and controls are investigated. METHODS: CD8+ T cells 
      were either stimulated non-specifically ex vivo and intracellular cytokines 
      examined, or cocultured with DC and other stimuli, for 2 weeks. The resulting 
      lines were analysed for cytokine expression. Clones derived from these lines were 
      tested for regulatory function. RESULTS: PBMCs from patients with AS and from 
      human leukocyte antigen (HLA)-B27+ healthy controls contained a higher frequency 
      of IL4+CD8+ T cells than those from HLA-B27- controls. Likewise, CD8+ T cell 
      lines obtained by coculture with DC contained a higher ratio of IL4+ to 
      interferon (IFN)gamma+ cells when obtained from patients with AS or HLA-B27+ 
      controls. T cell clones obtained from these lines showed regulatory activity. 
      Outgrowth of IL4+ CD8+ T cells required contact with DC, but not maturation with 
      lipopolysaccharide (LPS); allogeneic DC were also effective. Coculture with 
      lymphoblastoid cells, or anti-CD3/CD28 microbeads, produced only expansion of IFN 
      gamma-producing CD8+ T cells. CONCLUSIONS: The higher proportion of CD8+ cells 
      which can produce IL4 in PB and in expanded CD8+ T cell lines suggests an altered 
      pattern of CD8+ T cell differentiation in AS and in HLA-B27+ healthy individuals. 
      This predisposition to generate IL4+CD8+ T cells may play a role in pathogenesis 
      of spondyloarthritis.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Rheumatology and Immunology, Changzheng Hospital, Shanghai, China.
FAU - Jarvis, L B
AU  - Jarvis LB
FAU - Baek, H-J
AU  - Baek HJ
FAU - Gaston, J S Hill
AU  - Gaston JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080722
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (HLA-B27 Antigen)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Adult
MH  - Cell Communication/immunology
MH  - Cell Line
MH  - Coculture Techniques
MH  - Dendritic Cells/immunology
MH  - Female
MH  - HLA-B27 Antigen/blood
MH  - Humans
MH  - Interleukin-4/*biosynthesis
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - Spondylitis, Ankylosing/*immunology
MH  - T-Lymphocyte Subsets/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
EDAT- 2008/07/24 09:00
MHDA- 2009/09/04 06:00
CRDT- 2008/07/24 09:00
PHST- 2008/07/24 09:00 [pubmed]
PHST- 2009/09/04 06:00 [medline]
PHST- 2008/07/24 09:00 [entrez]
AID - ard.2008.088120 [pii]
AID - 10.1136/ard.2008.088120 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2009 Aug;68(8):1345-51. doi: 10.1136/ard.2008.088120. Epub 2008 
      Jul 22.