PMID- 18648085
OWN - NLM
STAT- MEDLINE
DCOM- 20081223
LR  - 20151119
IS  - 1096-0929 (Electronic)
IS  - 1096-0929 (Linking)
VI  - 106
IP  - 1
DP  - 2008 Nov
TI  - Do parabens have the ability to interfere with steroidogenesis?
PG  - 206-13
LID - 10.1093/toxsci/kfn148 [doi]
AB  - The effects of ethyl and butyl paraben on steroidogenesis were evaluated in rats 
      exposed in utero. Pregnant Wistar rats were dosed from gestational day (GD) 7 to 
      GD 21, followed by examination of the dams, and the fetuses. Additionally, both 
      parabens were tested in vitro in the H295R steroidogenesis assay and in the 
      T-screen assay, the later to test for their ability to act as thyroid hormone 
      receptor agonist or antagonist. In the in utero exposure toxicity study, neither 
      ethyl nor butyl paraben showed any treatment-related effects on testosterone 
      production, anogenital distance, or testicular histopathology. However, butyl 
      paraben caused a significant decrease in the mRNA expression level of estradiol 
      receptor-beta in fetal ovaries, and also significantly decreased the mRNA 
      expression of steroidogenic acute regulatory protein and peripheral 
      benzodiazepine receptor in the adrenal glands. In vitro butyl paraben increased 
      the proliferation of the GH3 cells in the T-Screen assay, thereby acting as a 
      weak thyroid hormone receptor agonist. In the adrenal H295R steroidogenesis assay 
      both ethyl and butyl paraben caused a significant increase in the progesterone 
      formation. Overall, the results indicate that butyl paraben might have the 
      ability to act as endocrine disruptor by interfering with the transport of 
      cholesterol to the mitochondrion, thereby interfering with steroidogenesis, but 
      also that the two tested parabens do not show clear endocrine disrupting 
      capabilities in our short-term in vivo experiment.
FAU - Taxvig, Camilla
AU  - Taxvig C
AD  - National Food Institute, Technical University of Denmark, Department of 
      Toxicology and Risk Assessment, DK-2860 Soborg, Denmark. camta@food.dtu.dk
FAU - Vinggaard, Anne Marie
AU  - Vinggaard AM
FAU - Hass, Ulla
AU  - Hass U
FAU - Axelstad, Marta
AU  - Axelstad M
FAU - Boberg, Julie
AU  - Boberg J
FAU - Hansen, Pernille Reimer
AU  - Hansen PR
FAU - Frederiksen, Hanne
AU  - Frederiksen H
FAU - Nellemann, Christine
AU  - Nellemann C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080722
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Parabens)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estradiol)
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 14255EXE39 (ethyl-p-hydroxybenzoate)
RN  - 3QPI1U3FV8 (butylparaben)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Adrenal Glands/*drug effects/embryology/metabolism
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endocrine Disruptors/*toxicity
MH  - Female
MH  - Gestational Age
MH  - Gonadal Steroid Hormones/*biosynthesis/blood
MH  - Male
MH  - Ovary/*drug effects/embryology/metabolism
MH  - Parabens/*toxicity
MH  - Phosphoproteins/genetics/metabolism
MH  - Pituitary Gland/drug effects/metabolism
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Progesterone/biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Estradiol/genetics/metabolism
MH  - Receptors, GABA-A/genetics/metabolism
MH  - Receptors, Thyroid Hormone/agonists/metabolism
MH  - Testis/*drug effects/embryology/metabolism
MH  - Testosterone/biosynthesis
EDAT- 2008/07/24 09:00
MHDA- 2008/12/24 09:00
CRDT- 2008/07/24 09:00
PHST- 2008/07/24 09:00 [pubmed]
PHST- 2008/12/24 09:00 [medline]
PHST- 2008/07/24 09:00 [entrez]
AID - kfn148 [pii]
AID - 10.1093/toxsci/kfn148 [doi]
PST - ppublish
SO  - Toxicol Sci. 2008 Nov;106(1):206-13. doi: 10.1093/toxsci/kfn148. Epub 2008 Jul 
      22.