PMID- 1864928
OWN - NLM
STAT- MEDLINE
DCOM- 19910906
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 29
IP  - 6
DP  - 1991 Jun
TI  - Evaluation of a recombinant human T-cell lymphotropic virus type I (HTLV-I) p21E 
      antibody detection enzyme immunoassay as a supplementary test in HTLV-I/II 
      antibody testing algorithms.
PG  - 1125-7
AB  - To evaluate the usefulness of a human T-cell lymphotropic virus type I (HTLV-I) 
      recombinant p21E immunoassay as a supplementary test in HTLV-I/II serologic 
      testing algorithms, we used this assay to test 378 serum samples previously 
      categorized as positive, indeterminant, or negative for HTLV-I/II antibody, as 
      defined by U.S. Public Health Service guidelines. We found this test to be highly 
      sensitive for detecting antibody to HTLV-I/II env (99.4%) but slightly less 
      specific (96.0%), particularly among samples from intravenous drug users. Our 
      data suggest that this test is most appropriately used to confirm the absence of 
      env antibody in samples which are repeatably reactive in an HTLV-I/II screening 
      assay and gag reactive only by immunoblotting. Because of the high sensitivity of 
      this recombinant p21E test, a negative result in this context could preclude 
      radioimmunoprecipitation testing. However, pending further evaluation of the 
      specificity of this assay, samples testing positive for p21 env antibody may 
      require confirmation by radioimmunoprecipitation, particularly in situations in 
      which the results will be used for diagnostic purposes or blood donor counseling.
FAU - Hartley, T M
AU  - Hartley TM
AD  - Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
FAU - Malone, G E
AU  - Malone GE
FAU - Khabbaz, R F
AU  - Khabbaz RF
FAU - Lal, R B
AU  - Lal RB
FAU - Kaplan, J E
AU  - Kaplan JE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Gene Products, env)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-II Antibodies)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gp21 protein, Human T-lymphotropic virus 1)
SB  - IM
MH  - Algorithms
MH  - Evaluation Studies as Topic
MH  - Gene Products, env/*immunology
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-II Antibodies/*blood
MH  - Human T-lymphotropic virus 1/immunology
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Retroviridae Proteins, Oncogenic/*immunology
MH  - env Gene Products, Human Immunodeficiency Virus
PMC - PMC269955
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
PMCR- 1991/10/01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.29.6.1125-1127.1991 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1991 Jun;29(6):1125-7. doi: 10.1128/jcm.29.6.1125-1127.1991.