PMID- 1865362
OWN - NLM
STAT- MEDLINE
DCOM- 19910911
LR  - 20151119
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 258
IP  - 2
DP  - 1991 Aug
TI  - Purified choleragenoid does not induce migrating action potential complex 
      activity in rabbit ileum in vivo.
PG  - 647-51
AB  - The migrating action potential complex (MAPC), a single ring contraction that 
      propels luminal contents abroad, is elicited by Vibrio cholerae and its 
      enterotoxin, choleragen (A1A2B5), in rabbit ileal loops in vivo. Choleragenoid 
      (B5; the binding subunit) was shown previously to induce MAPCs without activating 
      the adenylate cyclase system and without stimulating fluid output. We restudied 
      purified B5 (extracted by high-performance liquid chromatography) to assess its 
      effects on the myoelectric activity of rabbit ileum: can MAPC activity can be 
      induced without associated fluid production? Four different B5 preparations and 
      two controls, V. cholerae and sterile saline, were used. MAPC activity and ileal 
      fluid output were significantly increased in animals inoculated with A1A2B5 (V. 
      cholerae-El Tor) compared with all other preparations and controls. Importantly, 
      MAPC activity and fluid output in all B5 groups did not differ from those in 
      saline controls, demonstrating that purified B5 does not induce MAPC activity in 
      rabbit ilium in vivo. The previous study showing B5-induced MAPCs may have 
      resulted from A1A2B5 contamination. These results suggest A1A2B5-induced 
      adenylate cyclase activation; myoelectric activity may be interrelated with 
      holotoxin binding that causes a neural response.
FAU - Lind, C D
AU  - Lind CD
AD  - Department of Internal Medicine, University of Virginia, Charlottesville.
FAU - Guerrant, R L
AU  - Guerrant RL
FAU - Kurosky, A
AU  - Kurosky A
FAU - Mathias, J R
AU  - Mathias JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Adenylyl Cyclases/analysis
MH  - Animals
MH  - Cholera Toxin/isolation & purification/*pharmacology
MH  - Enzyme Activation
MH  - Ileum/*drug effects/physiology
MH  - In Vitro Techniques
MH  - Male
MH  - Rabbits
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 1991 Aug;258(2):647-51.