PMID- 18653972
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20210102
IS  - 1344-6304 (Print)
IS  - 1344-6304 (Linking)
VI  - 61
IP  - 4
DP  - 2008 Jul
TI  - Single-cell observation of phagocytosis by human blood dendritic cells.
PG  - 294-7
AB  - Time-lapse video microscopic observation is useful for analysis of cell biology, 
      especially in rapid response of immune cells. Dendritic cells (DCs) have multiple 
      functions in the immune system, and DCs in peripheral blood play an especially 
      important role at the front line of infection. We have developed a time-lapse 
      video microscopic method for the evaluation of single myeloid DCs (MDC-1) from 
      human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and 
      phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 
      increased following stimulation with P. aeruginosa but not after stimulation with 
      supernatant from a P. aeruginosa culture. The activation of these morphological 
      changes in MDC-1 could be quantitatively analyzed using the time-lapse video 
      microscopy. This novel system may be useful for the evaluation of rapid response 
      with human immune cells against bacterial infection.
FAU - Ishimoto, Hiroshi
AU  - Ishimoto H
AD  - Second Department of Internal Medicine, Nagasaki University School of Medicine, 
      Nagasaki 852-8501, Japan. kyana-ngs@umin.ac.jp.
FAU - Yanagihara, Katsunori
AU  - Yanagihara K
FAU - Araki, Nobuko
AU  - Araki N
FAU - Mukae, Hiroshi
AU  - Mukae H
FAU - Sakamoto, Noriho
AU  - Sakamoto N
FAU - Izumikawa, Koichi
AU  - Izumikawa K
FAU - Seki, Masafumi
AU  - Seki M
FAU - Miyazaki, Yoshitsugu
AU  - Miyazaki Y
FAU - Hirakata, Yoichi
AU  - Hirakata Y
FAU - Mizuta, Yohei
AU  - Mizuta Y
FAU - Yasuda, Kenji
AU  - Yasuda K
FAU - Kohno, Shigeru
AU  - Kohno S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Japan
TA  - Jpn J Infect Dis
JT  - Japanese journal of infectious diseases
JID - 100893704
RN  - 0 (CD11c Antigen)
SB  - IM
MH  - Antigen Presentation
MH  - CD11c Antigen/metabolism
MH  - Cell Differentiation
MH  - Dendritic Cells/cytology/*immunology/microbiology
MH  - Humans
MH  - Microscopy, Interference/*methods
MH  - Microscopy, Video/*methods
MH  - Myeloid Cells/cytology
MH  - Phagocytosis/*immunology
MH  - *Pseudomonas aeruginosa/growth & development
MH  - Time Factors
EDAT- 2008/07/26 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/07/26 09:00
PHST- 2008/07/26 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/07/26 09:00 [entrez]
PST - ppublish
SO  - Jpn J Infect Dis. 2008 Jul;61(4):294-7.