PMID- 18655176
OWN - NLM
STAT- MEDLINE
DCOM- 20090617
LR  - 20131121
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 24
IP  - 3
DP  - 2009 Jun
TI  - Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from 
      goldfish (Carassius auratus).
PG  - 225-34
LID - 10.1002/tox.20422 [doi]
AB  - We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish 
      (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino 
      acid protein with a theoretical molecular weight of 59.02 kDa. The CYP1A amino 
      acid sequence clusters in a monophyletic group with other fish CYP1As, and more 
      closely related to zebrafish CYP1A (91% identity) than to other fish CYP1As. 
      Exposure to benzo(a)pyrene (BaP) by intraperitoneal injection increased biliary 
      BaP metabolites and liver CYP1A gene expression. BaP exposure also increased 
      CYP1A gene expression in extrahepatic organs, including intestine, and gill, 
      which are sensitive to aqueous and dietary exposure to Arylhydrocarbon receptor 
      (AhR) agonists. Therefore, goldfish CYP1A identified in this study offers basic 
      information for further research related to biomarker use of CYP1A of goldfish.
FAU - Oh, Seung-Min
AU  - Oh SM
AD  - College of Pharmacy, Sungkyunkwan University, #300, Cheoncheondong, Jangan-Gu, 
      Suwon, Gyeonggi-do 440-746, Korea.
FAU - Ryu, Byung Taek
AU  - Ryu BT
FAU - Kim, Ha Ryong
AU  - Kim HR
FAU - Choi, Kyunghee
AU  - Choi K
FAU - Chung, Kyu-Hyuck
AU  - Chung KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Benzo(a)pyrene/pharmacokinetics/*pharmacology
MH  - Bile/metabolism
MH  - Cloning, Molecular
MH  - Cytochrome P-450 CYP1A1/classification/*genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Goldfish/*genetics/metabolism
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2008/07/26 09:00
MHDA- 2009/06/18 09:00
CRDT- 2008/07/26 09:00
PHST- 2008/07/26 09:00 [pubmed]
PHST- 2009/06/18 09:00 [medline]
PHST- 2008/07/26 09:00 [entrez]
AID - 10.1002/tox.20422 [doi]
PST - ppublish
SO  - Environ Toxicol. 2009 Jun;24(3):225-34. doi: 10.1002/tox.20422.