PMID- 18655198
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20240415
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 86
IP  - 15
DP  - 2008 Nov 15
TI  - Up-regulation of brain-derived neurotrophic factor by application of fibroblast 
      growth factor-2 to the cut optic nerve is important for long-term survival of 
      retinal ganglion cells.
PG  - 3382-92
LID - 10.1002/jnr.21793 [doi]
AB  - Application of basic fibroblast growth factor (FGF-2) to the optic nerve after 
      axotomy promotes the survival of retinal ganglion cells (RGCs) in the frog Rana 
      pipiens and results in a rapid up-regulation of brain-derived neurotrophic factor 
      (BDNF) and TrkB synthesis by the RGCs. Here we investigate whether this 
      up-regulation is maintained over the long term and whether it is required for 
      FGF-2's survival effect. At 6 weeks after axotomy and FGF-2 treatment, we found 
      more RGCs immunopositive for BDNF protein and higher intensity of BDNF and TrkB 
      immunostaining, accompanied by increases in BDNF and TrkB mRNA in RGCs. 
      Application of fluorescently labeled siRNA targeted against BDNF to the cut RGC 
      axons showed that it was transported to the cell bodies. Axonal siRNA treatment 
      eliminated the increases in BDNF immunostaining and mRNA that were induced by 
      FGF-2 and had no effect on TrkB mRNA. This reduction in BDNF synthesis by siRNA 
      greatly reduced the long-term survival effect of FGF-2 on RGCs. This, taken 
      together with previous results, suggests that, although FGF-2 may initially 
      activate survival pathways via ERK signaling, its main long-term survival effects 
      are mediated via its up-regulation of BDNF synthesis by the RGCs.
FAU - Blanco, Rosa E
AU  - Blanco RE
AD  - Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, Old 
      San Juan, Puerto Rico. rblanco@rcm.upr.edu
FAU - Soto, Ileana
AU  - Soto I
FAU - Duprey-Diaz, Mildred
AU  - Duprey-Diaz M
FAU - Blagburn, Jonathan M
AU  - Blagburn JM
LA  - eng
GR  - G12 RR003051/RR/NCRR NIH HHS/United States
GR  - S06 GM008224/GM/NIGMS NIH HHS/United States
GR  - S06 GM08224/GM/NIGMS NIH HHS/United States
GR  - G12RR-03051/RR/NCRR NIH HHS/United States
GR  - G12 MD007600/MD/NIMHD NIH HHS/United States
GR  - S06 GM008224-230039/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Survival/physiology
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Optic Nerve/*metabolism/pathology
MH  - RNA, Messenger/analysis
MH  - RNA, Small Interfering
MH  - Rana pipiens
MH  - Receptor, trkB/metabolism
MH  - Retinal Ganglion Cells/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Up-Regulation
PMC - PMC2587377
MID - NIHMS55923
EDAT- 2008/07/26 09:00
MHDA- 2009/01/23 09:00
PMCR- 2009/11/15
CRDT- 2008/07/26 09:00
PHST- 2008/07/26 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PHST- 2008/07/26 09:00 [entrez]
PHST- 2009/11/15 00:00 [pmc-release]
AID - 10.1002/jnr.21793 [doi]
PST - ppublish
SO  - J Neurosci Res. 2008 Nov 15;86(15):3382-92. doi: 10.1002/jnr.21793.