PMID- 18655886
OWN - NLM
STAT- MEDLINE
DCOM- 20090213
LR  - 20181201
IS  - 1875-7855 (Electronic)
IS  - 0079-6123 (Linking)
VI  - 170
DP  - 2008
TI  - Neurophysiology of supraoptic neurons in C57/BL mice studied in three acute in 
      vitro preparations.
PG  - 229-42
LID - 10.1016/S0079-6123(08)00420-2 [doi]
AB  - Osmotic control of arginine vasopressin (AVP) and oxytocin (OXT) release from 
      magnocellular neurosecretory cells (MNCs) of the supraoptic (SON) and 
      paraventricular (PVN) nuclei is essential for body fluid homeostasis. The 
      electrical activity of MNCs, which is regulated by intrinsic and extrinsic 
      osmosensitive factors, is a primary determinant of blood AVP and OXT levels. 
      Although we now understand many of the cellular mechanisms that mediate the 
      osmotic control of electrical activity and secretion from MNCs, further insight 
      is likely to emerge from a molecular analysis of these mechanisms. An important 
      step towards this goal could be made through the use of mouse genetic models. 
      However, the electrophysiological properties of MNCs in mice have not been 
      characterized, making direct comparisons with the rat model somewhat difficult. 
      In this study, we examined the electrical properties of MNCs from the mouse SON. 
      Extracellular recordings from neurons in superfused explants revealed modes of 
      basal and osmotically modulated firing very similar to those observed previously 
      in rats. Recordings in hypothalamic slices confirmed that SON neurons receive 
      kynurenic-acid-sensitive excitatory synaptic inputs from the organum vasculosum 
      laminae terminalis (OVLT). Current-clamp recordings from acutely dissociated SON 
      neurons showed proportional changes in membrane cation conductance during changes 
      in fluid osmolality. We conclude, therefore, that MNCs in the mouse SON display 
      intrinsic osmosensitive properties and firing patterns that are very similar to 
      those reported in the rat. Mouse MNCs therefore represent a useful model for the 
      study of molecular factors contributing to the osmotic control of AVP and OXT 
      release.
FAU - Sharif-Naeini, Reza
AU  - Sharif-Naeini R
AD  - Center for Research in Neuroscience, Montreal General Hospital and McGill 
      University, Montreal, Quebec, Canada.
FAU - Ciura, Sorana
AU  - Ciura S
FAU - Stachniak, Tevye J
AU  - Stachniak TJ
FAU - Trudel, Eric
AU  - Trudel E
FAU - Bourque, Charles W
AU  - Bourque CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Prog Brain Res
JT  - Progress in brain research
JID - 0376441
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 50-56-6 (Oxytocin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/blood/*metabolism
MH  - Body Fluids/physiology
MH  - Calcium/physiology
MH  - Disease Models, Animal
MH  - Exocytosis
MH  - Homeostasis
MH  - Hypothalamus/physiology
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Genetic
MH  - Neurons/*physiology
MH  - Neurosecretory Systems/physiology
MH  - Oxytocin/blood/*metabolism
MH  - Supraoptic Nucleus/*physiology
MH  - Water-Electrolyte Balance/physiology
EDAT- 2008/07/29 09:00
MHDA- 2009/02/14 09:00
CRDT- 2008/07/29 09:00
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2009/02/14 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - S0079-6123(08)00420-2 [pii]
AID - 10.1016/S0079-6123(08)00420-2 [doi]
PST - ppublish
SO  - Prog Brain Res. 2008;170:229-42. doi: 10.1016/S0079-6123(08)00420-2.