PMID- 18656687
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20131121
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 185
IP  - 1
DP  - 2008 Aug
TI  - Amplicons on chromosome 3 contain oncogenes induced by recurrent exposure to 
      12-O-tetradecanoylphorbol-13-acetate and sodium n-butyrate and Epstein-Barr virus 
      reactivation in a nasopharyngeal carcinoma cell line.
PG  - 1-10
LID - 10.1016/j.cancergencyto.2008.03.014 [doi]
AB  - Nasopharyngeal carcinoma (NPC) is closely associated with Epstein-Barr virus 
      (EBV) infection and exposure to environmental carcinogens. In this study, an 
      inducible Epstein-Barr virus (EBV) reactivation NPC cell line, NA, was used to 
      investigate the impact of recurrent 12-O-tetradecanoylphorbol-13-acetate-sodium 
      n-butyrate (TPA/SB) treatment and EBV reactivation on chromosomal abnormalities 
      utilizing array-based comparative genomic hybridization (CGH). It was observed 
      that most copy-number aberrations (CNA) were progressively nonrandomly clustered 
      on chromosomes 3, 8, and 9, as the frequency of TPA/SB treatment and EBV 
      reactivation increased. All of the prominent amplicons detected (including 
      3p14.1, 3p13, 3p12.3, 3p12.2, 3q26.2, 3q26.31, and 3q26.32) were located on 
      chromosome 3, with multiple oncogenes assigned to these sites. The amplification 
      patterns of 3p12.3 and 3q26.2 were validated using fluorescence in situ 
      hybridization (FISH) analysis. Subsequent quantitative real-time polymerase chain 
      reaction detected increasing expression of ROBO1 and SKIL oncogenes in NA cells 
      harboring higher frequency of TPA/SB treatment and EBV reactivation, consistent 
      with copy-number amplification of these loci. These findings demonstrate that a 
      high incidence of TPA/SB induced-EBV reactivation has a profound influence on the 
      carcinogenesis of NPC through altered DNA copy number.
FAU - Lee, Chia-Huei
AU  - Lee CH
AD  - National Institute of Cancer Research, National Health Research Institutes, R2, 
      R1211, Keyan Road, Zhunan Town, Miaoli County 350, Taiwan.
FAU - Fang, Chih-Yeu
AU  - Fang CY
FAU - Sheu, Jim Jinn-Chyuan
AU  - Sheu JJ
FAU - Chang, Yao
AU  - Chang Y
FAU - Takada, Kenzo
AU  - Takada K
FAU - Chen, Jen-Yang
AU  - Chen JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Butyrates)
RN  - 0 (Carcinogens)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Butyrates/*pharmacology
MH  - Carcinogens/pharmacology
MH  - Carcinoma/pathology/virology
MH  - Cell Line, Tumor
MH  - *Chromosomes, Human, Pair 3
MH  - Herpesvirus 4, Human/*genetics/physiology
MH  - Humans
MH  - Nasopharyngeal Neoplasms/pathology/*virology
MH  - *Oncogenes
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Virus Activation/drug effects
EDAT- 2008/07/29 09:00
MHDA- 2008/08/16 09:00
CRDT- 2008/07/29 09:00
PHST- 2007/11/14 00:00 [received]
PHST- 2008/03/24 00:00 [revised]
PHST- 2008/03/28 00:00 [accepted]
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - S0165-4608(08)00233-1 [pii]
AID - 10.1016/j.cancergencyto.2008.03.014 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Aug;185(1):1-10. doi: 
      10.1016/j.cancergencyto.2008.03.014.