PMID- 18656704
OWN - NLM
STAT- MEDLINE
DCOM- 20081009
LR  - 20190816
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 213
IP  - 7
DP  - 2008
TI  - Differential migration of human monocyte-derived dendritic cells after infection 
      with prevalent clinical strains of Mycobacterium tuberculosis.
PG  - 567-75
LID - 10.1016/j.imbio.2008.01.007 [doi]
AB  - Dendritic cells (DCs) play a key role in the host immune response to infections. 
      Mycobacterium tuberculosis (MTB) can inhibit the maturation of DCs and impair 
      their ability to stimulate T cell proliferation. Here, we assessed in vitro 
      migratory behavior of human monocyte-derived DCs (MoDC) when infected with 
      various MTB strains (H37Rv and prevalent clinical strains S7 and S10 from South 
      India). The migration of Rv and S7 infected MoDC towards secondary lymphoid 
      chemokine (CCL21) was 50% lower after 1 day of infection compared to LPS 
      stimulation. This reduced cell migration may be due to a block in the chemokine 
      receptor switch from CCR5 to CCR7 expression on MoDC. Only clinical strain S10 
      infected MoDC showed an up-regulation of CCR7 and down-regulation of CCR5 
      expression, similar to LPS stimulated MoDC. While Rv and S7 infected MoDC did not 
      display any alteration in expression of these receptors. Similarly, Rv and S7 
      infected MoDC did not induce IL-8, IP-10 and MCP-1 chemokine production. This 
      reduction in chemokine levels was reflected in the reduced chemoattraction of 
      CD4(+) T cells also. These findings suggest that there is variation in the 
      stimulation of MoDC with different clinical strains of MTB and this variation may 
      be dependent upon the virulence of the strain.
FAU - Rajashree, P
AU  - Rajashree P
AD  - Department of Immunology, Tuberculosis Research Centre (ICMR), Mayor VR 
      Ramanathan Road, Chetpet, Chennai 600031, India.
FAU - Supriya, P
AU  - Supriya P
FAU - Das, Sulochana D
AU  - Das SD
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080304
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Interleukin-8)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CXCL10/biosynthesis
MH  - *Chemotaxis
MH  - Dendritic Cells/*microbiology/*physiology
MH  - Down-Regulation
MH  - Humans
MH  - Interleukin-8/biosynthesis
MH  - Middle Aged
MH  - Monocytes
MH  - Mycobacterium tuberculosis/immunology/*pathogenicity
MH  - Receptors, CCR5/metabolism
MH  - Receptors, CCR7/metabolism
MH  - Receptors, Chemokine/*metabolism
MH  - Up-Regulation
MH  - Virulence
EDAT- 2008/07/29 09:00
MHDA- 2008/10/10 09:00
CRDT- 2008/07/29 09:00
PHST- 2007/10/09 00:00 [received]
PHST- 2008/01/04 00:00 [revised]
PHST- 2008/01/09 00:00 [accepted]
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2008/10/10 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - S0171-2985(08)00025-9 [pii]
AID - 10.1016/j.imbio.2008.01.007 [doi]
PST - ppublish
SO  - Immunobiology. 2008;213(7):567-75. doi: 10.1016/j.imbio.2008.01.007. Epub 2008 
      Mar 4.