PMID- 18656921
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20231213
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 56
IP  - 16
DP  - 2008 Aug 27
TI  - Inhibitory action of soybean beta-conglycinin hydrolysates on Salmonella 
      typhimurium translocation in Caco-2 epithelial cell monolayers.
PG  - 7522-7
LID - 10.1021/jf8012508 [doi]
AB  - Soybean protein hydrolysates are widely used as functional foods as they have 
      antioxidative properties able to enhance immune responses in humans. The alcalase 
      enzymatic hydrolysates of beta-conglycinin were fractionated by ultrafiltration, 
      and two main fractions, SP1 (<10 kDa) and SP2 (10-20 kDa), were obtained. The 
      effects of these two fractions on the growth, development of epithelial cells, 
      and formation of intercellular tight junctions were tested on an in vitro Caco-2 
      cell culture system. The inhibitory effects of SP1 and SP2 on the penetration of 
      Salmonella typhimurium into Caco-2 epithelial cells were also examined. The 
      results showed that the addition of >0.05 g/L of SP2 improved epithelial cell 
      growth and that a concentration of 0.5 g/L of SP2 increased intercellular tight 
      junction formation, which resulted in increased of transepithelial monolayer 
      resistance (TER) values. Moreover, a lower S. typhimurium count compared to 
      control was obtained when Caco-2 cells were grown in 0.05 and 0.5 g/L of SP2. 
      These results show that beta-conglycinin hydrolysates play an important role in 
      resisting S. typhimurium penetration into intestinal epithelial cells and that 
      high molecular mass peptides (10-20 kDa) were more effective overall than low 
      molecular mass peptides.
FAU - Yang, Baichong
AU  - Yang B
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, 17 Qinghua Donglu, Haidian District, Beijing 100083, China.
FAU - Lv, Ying
AU  - Lv Y
FAU - Chen, Yang
AU  - Chen Y
FAU - Wang, Jin
AU  - Wang J
FAU - Tang, Wuxia
AU  - Tang W
FAU - Guo, Shuntang
AU  - Guo S
LA  - eng
PT  - Journal Article
DEP - 20080726
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antigens, Plant)
RN  - 0 (Globulins)
RN  - 0 (Seed Storage Proteins)
RN  - 0 (Soybean Proteins)
RN  - 0 (beta-conglycinin protein, Glycine max)
RN  - EC 3.4.21.- (Subtilisins)
SB  - IM
MH  - Antigens, Plant
MH  - Bacterial Translocation/*drug effects
MH  - Caco-2 Cells
MH  - Globulins/metabolism/*pharmacology
MH  - Humans
MH  - Hydrolysis
MH  - Intestines/microbiology/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Salmonella typhimurium/*physiology
MH  - Seed Storage Proteins
MH  - Soybean Proteins/metabolism/*pharmacology
MH  - Glycine max/*chemistry
MH  - Subtilisins/metabolism
EDAT- 2008/07/29 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/07/29 09:00
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - 10.1021/jf8012508 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2008 Aug 27;56(16):7522-7. doi: 10.1021/jf8012508. Epub 2008 
      Jul 26.