PMID- 18657578
OWN - NLM
STAT- MEDLINE
DCOM- 20081113
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1783
IP  - 10
DP  - 2008 Oct
TI  - Novel function of neuron-restrictive silencer factor (NRSF) for 
      posttranscriptional regulation.
PG  - 1835-46
LID - 10.1016/j.bbamcr.2008.06.019 [doi]
AB  - The neuron-restrictive silencer factor (NRSF) functions as a transcriptional 
      repressor of neuronal genes in nonneuronal cells. However, it is expressed in 
      certain mature neurons in adults, suggesting that it might have complex and novel 
      roles depending on its cellular and physiological context. Overexpression of NRSF 
      led to both increased opioid ligand-binding activity of the endogenous MOR and 
      MOR-GFP fusion protein expression. In RNA immunoprecipitation and gel-shift 
      assays, NRSF specifically interacted with the NRSE sequence of MOR mRNA. When MOR 
      and NRSF genes were coexpressed, the specific ligand-binding activity of MOR was 
      increased in neuroblastoma NMB cells, but decreased in PC12 cells result from its 
      localization. Indeed, after overexpressing NRSF in NMB cells, the target RNA 
      moved to the translationally active polysomal fraction. Overexpression of NRSF 
      also led to enhanced phosphorylation of eIF4G. In contrast, knockdown of NRSF by 
      siRNA transfection significantly decreased eIF4G phosphorylation. These findings 
      indicate that NRSF may deliver the target MOR transcripts to the polyribosomal 
      complex and activate eIF4G phosphorylation, resulting in translational 
      activation. We report here a novel function of NRSF that enhance the translation 
      of the mu opioid receptor (MOR) gene through its RNA binding sequence, the 
      neuron-restrictive silencer element (NRSE).
FAU - Kim, Chun Sung
AU  - Kim CS
AD  - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, 
      Minnesota 55455, USA. cskim2@chosun.ac.kr
FAU - Hwang, Cheol Kyu
AU  - Hwang CK
FAU - Song, Kyu Young
AU  - Song KY
FAU - Choi, Hack Sun
AU  - Choi HS
FAU - Kim, Do Kyung
AU  - Kim DK
FAU - Law, Ping-Yee
AU  - Law PY
FAU - Wei, Li-Na
AU  - Wei LN
FAU - Loh, Horace H
AU  - Loh HH
LA  - eng
GR  - DA000564/DA/NIDA NIH HHS/United States
GR  - DA001583/DA/NIDA NIH HHS/United States
GR  - DA011190/DA/NIDA NIH HHS/United States
GR  - DA011806/DA/NIDA NIH HHS/United States
GR  - DA013926/DA/NIDA NIH HHS/United States
GR  - K05-DA070554/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080708
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Ligands)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Opioid, mu)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cytoplasm/metabolism
MH  - Gene Expression Regulation/*genetics
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Ligands
MH  - Phosphorylation
MH  - Protein Biosynthesis
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Receptors, Opioid, mu/metabolism
MH  - Repressor Proteins/genetics/*metabolism
EDAT- 2008/07/29 09:00
MHDA- 2008/11/14 09:00
CRDT- 2008/07/29 09:00
PHST- 2008/01/04 00:00 [received]
PHST- 2008/06/19 00:00 [revised]
PHST- 2008/06/20 00:00 [accepted]
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2008/11/14 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - S0167-4889(08)00243-7 [pii]
AID - 10.1016/j.bbamcr.2008.06.019 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2008 Oct;1783(10):1835-46. doi: 
      10.1016/j.bbamcr.2008.06.019. Epub 2008 Jul 8.