PMID- 18660793
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 22
IP  - 1
DP  - 2009 Jan
TI  - Epidermal growth factor receptor protein expression and gene amplification in the 
      chemorefractory metastatic embryonal carcinoma.
PG  - 7-12
LID - 10.1038/modpathol.2008.133 [doi]
AB  - Testicular cancer is the most common cancer in young male patients. The 
      combination of cisplatin-based chemotherapy and surgery has resulted in high cure 
      rates. However, a small percentage of patients have cancers that are refractory 
      to chemotherapy; treatment options for these patients are limited, and their 
      prognosis is generally poor. Further studies are needed to explore the molecular 
      pathogenesis pathways and potential targets of therapy for this group of highly 
      aggressive tumors. We analyzed 21 patients who presented with metastatic 
      embryonal carcinoma, were treated with chemotherapy, and subsequently underwent 
      retroperitoneal lymph node dissection. Immunostaining for epidermal growth factor 
      receptor (EGFR) was performed on paraffin-embedded tissue sections containing 
      tumor from these specimens, using the avidin-biotin-peroxidase method. EGFR gene 
      amplification was performed by interphase fluorescence in situ hybridization 
      (FISH). Immunohistochemically, 9 of 21 cases (43%) demonstrated positive EGFR 
      staining; 12 of 21 cases (57%) had absent or very limited EGFR expression. FISH 
      revealed EGFR amplification in 1 case (5%), polysomy in 15 of 21 cases (71%), and 
      normal disomy pattern in 5 of 21 cases (24%). A significant correlation between 
      EGFR protein expression level and its chromosome polysomy/amplification was 
      established (P=0.02). Our study showed that EGFR protein is frequently expressed 
      in a subset of patients with chemorefractory metastatic embryonal carcinoma. EGFR 
      chromosomal polysomy/amplification may be one of the mechanisms that cause 
      increased EGFR protein expression, and could potentially be used as indication 
      for anti-EGFR therapy.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN 46202, USA.
FAU - Zhang, Shaobo
AU  - Zhang S
FAU - Maclennan, Gregory T
AU  - Maclennan GT
FAU - Biermann, Katharina
AU  - Biermann K
FAU - Foster, Richard S
AU  - Foster RS
FAU - Beck, Stephen D
AU  - Beck SD
FAU - Cheng, Liang
AU  - Cheng L
LA  - eng
PT  - Journal Article
DEP - 20080725
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Carcinoma, Embryonal/*genetics/metabolism/pathology
MH  - Drug Resistance, Neoplasm/*genetics
MH  - ErbB Receptors/*genetics/metabolism
MH  - *Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm, Residual/genetics/metabolism/pathology
MH  - Retrospective Studies
MH  - Testicular Neoplasms/*genetics/metabolism/pathology
EDAT- 2008/07/29 09:00
MHDA- 2009/03/04 09:00
CRDT- 2008/07/29 09:00
PHST- 2008/07/29 09:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2008/07/29 09:00 [entrez]
AID - S0893-3952(22)02579-0 [pii]
AID - 10.1038/modpathol.2008.133 [doi]
PST - ppublish
SO  - Mod Pathol. 2009 Jan;22(1):7-12. doi: 10.1038/modpathol.2008.133. Epub 2008 Jul 
      25.