PMID- 18662029
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20211203
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 9
IP  - 9
DP  - 2008 Sep
TI  - Ring-shaped assembly of microtubules shows preferential counterclockwise motion.
PG  - 2277-82
LID - 10.1021/bm800639w [doi]
AB  - In this paper, we reveal that microtubules (MTs), reconstructed from tubulin in 
      vitro in the presence of guanosine-5'-triphosphate (GTP), have a ring or spiral 
      shape on a motor protein-fixed surface, and these MTs show biased motion in the 
      counterclockwise direction. By cross-linking these MTs during the sliding motion, 
      we obtained large ring-shaped MT assemblies, 1 approximately 12.6 microm in 
      diameter. The ratio of the rings rotating in the counterclockwise direction to 
      those rotating in the clockwise direction was approximately 3/1. Under optimized 
      conditions, the ratio was as high as 14/1. Thus, we successfully obtained 
      aggregated MTs with a large hierarchic structure that shows a preferential 
      motion, through a dynamic process in vitro.
FAU - Kawamura, Ryuzo
AU  - Kawamura R
AD  - Division of Biological Sciences, Graduate School of Science, Hokkaido University, 
      Sapporo 060-0810, Japan.
FAU - Kakugo, Akira
AU  - Kakugo A
FAU - Shikinaka, Kazuhiro
AU  - Shikinaka K
FAU - Osada, Yoshihito
AU  - Osada Y
FAU - Gong, Jian Ping
AU  - Gong JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080729
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Rhodamines)
RN  - 0 (Tubulin)
RN  - 6SO6U10H04 (Biotin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Biotin/chemistry
MH  - Guanosine Triphosphate/metabolism
MH  - Kinesins/isolation & purification/metabolism
MH  - Microtubules/*chemistry/*metabolism
MH  - *Movement
MH  - Particle Size
MH  - Rhodamines/chemistry
MH  - Rotation
MH  - Staining and Labeling
MH  - Surface Properties
MH  - Time Factors
MH  - Tubulin/chemistry/isolation & purification/metabolism
EDAT- 2008/07/30 09:00
MHDA- 2009/01/27 09:00
CRDT- 2008/07/30 09:00
PHST- 2008/07/30 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2008/07/30 09:00 [entrez]
AID - 10.1021/bm800639w [doi]
PST - ppublish
SO  - Biomacromolecules. 2008 Sep;9(9):2277-82. doi: 10.1021/bm800639w. Epub 2008 Jul 
      29.