PMID- 18662786
OWN - NLM
STAT- MEDLINE
DCOM- 20081231
LR  - 20081017
IS  - 1096-0279 (Electronic)
IS  - 1046-5928 (Linking)
VI  - 62
IP  - 1
DP  - 2008 Nov
TI  - Expression and purification of recombinant human interleukin-18 protein using a 
      yeast expression system.
PG  - 44-8
LID - 10.1016/j.pep.2008.06.013 [doi]
AB  - Interleukin-18 (IL-18) has been reported to exert significant immunoregulatory 
      effects on inhibiting tumor growth through stimulating natural killer (NK) cell 
      cytotoxicity and promoting production of several cytokines, including 
      interferon-gamma (IFN-gamma) and granulocyte/macrophage colony-stimulating factor 
      (GM-CSF). Therefore, IL-18 might serve as a potential therapeutic target for 
      cancer treatment. However, the resource of this protein limits its availability 
      for the clinical practice. The purpose of this study was to express and purify 
      recombinant human (h) IL-18 protein using a yeast expression system. We reported 
      here that hIL-18 gene was cloned into pPICZaC vector for expressing a recombinant 
      hIL-18 protein using a yeast expression system. The recombinant hIL-18 protein 
      was purified using centrifugal filter devices, hydrophobic chromatography, and 
      anion exchange chromatography. The yield and purity of the recombinant hIL-18 
      reached 45.1% and 97.6%, respectively. This recombinant hIL-18 was shown to 
      induce IFN-gamma production by human peripheral blood mononuclear cells (PBMCs) 
      and enhance NK cell cytotoxicity synergistically with IL-2. Furthermore, these 
      recombinant hIL-18-induced effects were the same as those by standard hIL-18. 
      Therefore, the yeast expression system used in this study provides a useful 
      method to produce large-scale of hIL-18 for the clinical application.
FAU - Yang, Lili
AU  - Yang L
AD  - Department of Immunology, Key Laboratory of Cancer Prevention and Therapy, 
      Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tiyuanbei, 
      Hexi District, Tianjin 300060, PR China.
FAU - An, Xiumei
AU  - An X
FAU - Wei, Feng
AU  - Wei F
FAU - Liu, Hong
AU  - Liu H
FAU - Li, Hui
AU  - Li H
FAU - Yu, Jinpu
AU  - Yu J
FAU - Ren, Xiubao
AU  - Ren X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080708
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Gene Expression
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-18/*genetics/*isolation & purification/metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Pichia/*genetics/metabolism
MH  - Recombinant Proteins/*genetics/*isolation & purification/metabolism
EDAT- 2008/07/30 09:00
MHDA- 2009/01/01 09:00
CRDT- 2008/07/30 09:00
PHST- 2008/05/10 00:00 [received]
PHST- 2008/06/27 00:00 [revised]
PHST- 2008/06/27 00:00 [accepted]
PHST- 2008/07/30 09:00 [pubmed]
PHST- 2009/01/01 09:00 [medline]
PHST- 2008/07/30 09:00 [entrez]
AID - S1046-5928(08)00175-7 [pii]
AID - 10.1016/j.pep.2008.06.013 [doi]
PST - ppublish
SO  - Protein Expr Purif. 2008 Nov;62(1):44-8. doi: 10.1016/j.pep.2008.06.013. Epub 
      2008 Jul 8.