PMID- 18665625
OWN - NLM
STAT- MEDLINE
DCOM- 20081104
LR  - 20151119
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 7
IP  - 9
DP  - 2008 Sep
TI  - Fine-needle aspiration of thyroid nodules: proteomic analysis to identify cancer 
      biomarkers.
PG  - 4079-88
LID - 10.1021/pr8000404 [doi]
AB  - At present, the clinical and pathological analysis used in the diagnosis of 
      papillary thyroid cancer (PTC) are insufficient to discern tumor behavior, and 
      new diagnostic and prognostic markers need to be identified. In this study, we 
      performed a comparative proteome analysis to examine the global changes of fine 
      needle aspiration fluid (FNA) protein patterns of two variants of malignant PTC 
      (classical variant PTC (cPTC) and tall cell variant PTC (TCV)) with respect to 
      the controls. Changes in protein expression were identified using two-dimensional 
      electrophoresis (2DE) and peptide mass fingerprinting via MALDI-TOF mass 
      spectrometry (MS), as well as Western blot analysis. A statistical significant 
      up-regulation of 17 protein spots in cPTC and/or TCV with respect to controls was 
      demonstrated. These proteins included transthyretin precursor (TTR), ferritin 
      light chain (FLC), proteasome activator complex subunit 1 and 2, 
      alpha-1-antitrypsin precursor, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 
      lactate dehydrogenase chain B (LDH-B), apolipoprotein A1 precursor (Apo-A1), 
      annexin A1, DJ-1 protein and cofilin-1. In addition, 12 protein spots were found 
      exclusively in cPTC and three exclusively in TCV. These latter proteins (ferritin 
      heavy chain (FHC), peroxiredoxin 1 (PRX1) and 6-phosphogluconate dehydrogenase 
      (6-PDGH)) correspond to stress response proteins and, until now, had not been 
      described in thyroid tumors. These findings illustrate the potential use of FNA 
      proteomics to identify protein changes associated with thyroid cancer and to 
      advance potential protein biomarkers in the diagnostic classification of the 
      disease.
FAU - Giusti, Laura
AU  - Giusti L
AD  - Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, 
      University of Pisa, Pisa, Italy.
FAU - Iacconi, Pietro
AU  - Iacconi P
FAU - Ciregia, Federica
AU  - Ciregia F
FAU - Giannaccini, Gino
AU  - Giannaccini G
FAU - Donatini, Gian Luca
AU  - Donatini GL
FAU - Basolo, Fulvio
AU  - Basolo F
FAU - Miccoli, Paolo
AU  - Miccoli P
FAU - Pinchera, Aldo
AU  - Pinchera A
FAU - Lucacchini, Antonio
AU  - Lucacchini A
LA  - eng
PT  - Journal Article
DEP - 20080730
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proteome)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Biopsy, Needle
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Proteome
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Thyroid Neoplasms/metabolism/*pathology
EDAT- 2008/07/31 09:00
MHDA- 2008/11/05 09:00
CRDT- 2008/07/31 09:00
PHST- 2008/07/31 09:00 [pubmed]
PHST- 2008/11/05 09:00 [medline]
PHST- 2008/07/31 09:00 [entrez]
AID - 10.1021/pr8000404 [doi]
PST - ppublish
SO  - J Proteome Res. 2008 Sep;7(9):4079-88. doi: 10.1021/pr8000404. Epub 2008 Jul 30.