PMID- 18667067
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 1745-6673 (Electronic)
IS  - 1745-6673 (Linking)
VI  - 3
DP  - 2008 Jul 30
TI  - Lipopolysaccharide induced inflammation in the perivascular space in lungs.
PG  - 17
LID - 10.1186/1745-6673-3-17 [doi]
AB  - BACKGROUND: Lipopolysaccharide (LPS) contained in tobacco smoke and a variety of 
      environmental and occupational dusts is a toxic agent causing lung inflammation 
      characterized by migration of neutrophils and monocytes into alveoli. Although 
      migration of inflammatory cells into alveoli of LPS-treated rats is well 
      characterized, the dynamics of their accumulation in the perivascular space (PVS) 
      leading to a perivascular inflammation (PVI) of pulmonary arteries is not well 
      described. METHODS: Therefore, we investigated migration of neutrophils and 
      monocytes into PVS in lungs of male Sprague-Dawley rats treated intratracheally 
      with E. coli LPS and euthanized after 1, 6, 12, 24 and 36 hours. Control rats 
      were treated with endotoxin-free saline. H&E stained slides were made and 
      immunohistochemistry was performed using a monocyte marker and the chemokine 
      Monocyte-Chemoattractant-Protein-1 (MCP-1). Computer-assisted microscopy was 
      performed to count infiltrating cells. RESULTS: Surprisingly, the periarterial 
      infiltration was not a constant finding in each animal although LPS-induced 
      alveolitis was present. A clear tendency was observed that neutrophils were 
      appearing in the PVS first within 6 hours after LPS application and were 
      decreasing at later time points. In contrast, mononuclear cell infiltration was 
      observed after 24 hours. In addition, MCP-1 expression was present in 
      perivascular capillaries, arteries and the epithelium. CONCLUSION: PVI might be a 
      certain lung reaction pattern in the defense to infectious attacks.
FAU - Tschernig, Thomas
AU  - Tschernig T
AD  - Dept. of Functional and Applied Anatomy -4120-, Medical School of Hannover, 
      Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
FAU - Janardhan, Kyathanahalli S
AU  - Janardhan KS
AD  - Immunology Research Group, Departments of Veterinary Biomedical Sciences and 
      Veterinary Microbiology, Western College of Veterinary Medicine, University of 
      Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada.
AD  - Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Kansas State 
      University, Manhattan, Kansas 66506, USA.
FAU - Pabst, Reinhard
AU  - Pabst R
AD  - Dept. of Functional and Applied Anatomy -4120-, Medical School of Hannover, 
      Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
FAU - Singh, Baljit
AU  - Singh B
AD  - Immunology Research Group, Departments of Veterinary Biomedical Sciences and 
      Veterinary Microbiology, Western College of Veterinary Medicine, University of 
      Saskatchewan, 52 Campus Drive, Saskatoon, SK, S7N 5B4, Canada.
LA  - eng
PT  - Journal Article
DEP - 20080730
PL  - England
TA  - J Occup Med Toxicol
JT  - Journal of occupational medicine and toxicology (London, England)
JID - 101245790
PMC - PMC2518552
EDAT- 2008/08/01 09:00
MHDA- 2008/08/01 09:01
PMCR- 2008/07/30
CRDT- 2008/08/01 09:00
PHST- 2007/03/29 00:00 [received]
PHST- 2008/07/30 00:00 [accepted]
PHST- 2008/08/01 09:00 [pubmed]
PHST- 2008/08/01 09:01 [medline]
PHST- 2008/08/01 09:00 [entrez]
PHST- 2008/07/30 00:00 [pmc-release]
AID - 1745-6673-3-17 [pii]
AID - 10.1186/1745-6673-3-17 [doi]
PST - epublish
SO  - J Occup Med Toxicol. 2008 Jul 30;3:17. doi: 10.1186/1745-6673-3-17.