PMID- 18667217 OWN - NLM STAT- MEDLINE DCOM- 20090609 LR - 20131121 IS - 0034-5288 (Print) IS - 0034-5288 (Linking) VI - 86 IP - 2 DP - 2009 Apr TI - Relationships between oxidative stress markers and red blood cell characteristics in renal azotemic dogs. PG - 309-13 LID - 10.1016/j.rvsc.2008.06.003 [doi] AB - Oxidative stress parameters and erythrocyte characteristics were studied in 15 normal healthy dogs and 33 renal azotaemic dogs from Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. Dogs with renal azotaemia had reduced mean corpuscular volume (MCV) (P<0.01), packed cell volume (PCV) (P<0.001) and increased mean corpuscular hemoglobin concentration (MCHC) (P<0.001). The relationship was found between degree of azotaemia and MCV, PCV and MCHC. Dogs with severe renal azotaemia had higher intraerythrocytic sodium contents (RBC-Na) (P<0.05). The red blood cell catalase activity and glutathione and plasma malondialdehyde were unaltered while urinary malondialdehyde-creatinine ratio (U-MDA/Cr) increased significantly (P<0.001). The U-MDA/Cr was correlated significantly with plasma creatinine concentration (P<0.05), urinary protein-creatinine ratio (P<0.05) and fractional excretion of sodium (P<0.001). The results suggest some changes in RBC characteristics and urine oxidative stress marker in renal azotaemic dogs. Moreover, the U-MDA/Cr is a sensitive biochemical parameter which increased along with degree of renal dysfunction. FAU - Buranakarl, C AU - Buranakarl C AD - Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. bchollad@chula.ac.th FAU - Trisiriroj, M AU - Trisiriroj M FAU - Pondeenana, S AU - Pondeenana S FAU - Tungjitpeanpong, T AU - Tungjitpeanpong T FAU - Jarutakanon, P AU - Jarutakanon P FAU - Penchome, R AU - Penchome R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080729 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 4Y8F71G49Q (Malondialdehyde) RN - 9NEZ333N27 (Sodium) RN - EC 1.11.1.6 (Catalase) RN - GAN16C9B8O (Glutathione) RN - RWP5GA015D (Potassium) SB - IM MH - Animals MH - Azotemia/blood/enzymology/metabolism/*veterinary MH - Blood Cell Count/veterinary MH - Catalase/blood MH - Dog Diseases/blood/enzymology/*metabolism MH - Dogs MH - Erythrocytes/enzymology/*metabolism/pathology MH - Female MH - Glutathione/blood MH - Hematocrit/veterinary MH - Kidney Diseases/blood/enzymology/metabolism/*veterinary MH - Male MH - Malondialdehyde/blood/urine MH - Osmotic Fragility MH - Oxidative Stress/*physiology MH - Potassium/blood MH - Sodium/blood EDAT- 2008/08/01 09:00 MHDA- 2009/06/10 09:00 CRDT- 2008/08/01 09:00 PHST- 2006/10/16 00:00 [received] PHST- 2008/04/25 00:00 [revised] PHST- 2008/06/10 00:00 [accepted] PHST- 2008/08/01 09:00 [pubmed] PHST- 2009/06/10 09:00 [medline] PHST- 2008/08/01 09:00 [entrez] AID - S0034-5288(08)00149-5 [pii] AID - 10.1016/j.rvsc.2008.06.003 [doi] PST - ppublish SO - Res Vet Sci. 2009 Apr;86(2):309-13. doi: 10.1016/j.rvsc.2008.06.003. Epub 2008 Jul 29.