PMID- 18667775
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20211203
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 33
IP  - 7
DP  - 2008 Jul
TI  - [Effeet of rapamycin on mTOR and eIF-4E expression in coxsackievirus B3-induced 
      rat myocardial cells].
PG  - 612-7
AB  - OBJECTIVE: To observe the effeet of rapamycin, an inhibitor of mammalian target 
      of rapamycin (mTOR), on mTOR and eukaryotic initiation 
      factor-4E(eIF-4E)expression in coxsac-kievirus B3 (CVB3)-induced rat myocardial 
      cells and to investigate the role of mTOR/eIF-4E signal pathway in viral 
      myocarditis. METHODS: To construct a cell model of viral myocarditis with primary 
      cultured myocardial cells. Myocardial cells infected by CVB3 were treated with 10 
      nmol/L rapamycin according to the cell toxicity test. The mTOR and eIF-4E 
      expressions of cells were determined by RT-PCR and Western Blot. RESULTS: 
      Rapamycin inhibited the degeneration of CVB3-induced myocardial cells. 
      Expressions of mTOR and eIF-4E mRNA or protein in CVB3-induced myocardial cells 
      were significantly upregulated compared with the control group (P < 0.05), and 
      rapamycin (10 nmol/L) inhibited the upregulation (P < 0.05). CONCLUSION: 
      Rapamycin can downregulate the expressions of mTOR and eIF-4E in CVB3-induced 
      myocardial cells, suggesting that mTOR/eIF-4E signal transduction may play an 
      important role in viral myocarditis.
FAU - Chen, Chun-Yuan
AU  - Chen CY
AD  - Department of Pediatics, Third Xiangya Hospital, Central South University, 
      Changsha 410013, China.
FAU - Sun, Yue-Nu
AU  - Sun YN
FAU - Yang, Zuo-Cheng
AU  - Yang ZC
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Coxsackievirus Infections
MH  - *Enterovirus B, Human
MH  - Eukaryotic Initiation Factor-4E/*biosynthesis
MH  - *Myocarditis/metabolism/virology
MH  - Myocytes, Cardiac/metabolism
MH  - Protein Kinases/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Sirolimus/*pharmacology
MH  - TOR Serine-Threonine Kinases
EDAT- 2008/08/01 09:00
MHDA- 2009/04/29 09:00
CRDT- 2008/08/01 09:00
PHST- 2008/08/01 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2008/08/01 09:00 [entrez]
AID - 1672-7347(2008)07-0612-06 [pii]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Jul;33(7):612-7.