PMID- 18670098
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20190720
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 31
IP  - 8
DP  - 2008 Aug
TI  - Inhibition of monocyte chemoattractant protein-1 by Kruppel-like factor 5 small 
      interfering RNA in the tumor necrosis factor- alpha-activated human umbilical 
      vein endothelial cells.
PG  - 1609-13
AB  - Kruppel-like factor 5 (KLF5) is one of the pivotal transcriptional factors 
      communicating with inflammatory cytokines. Regulation of monocyte chemoattractant 
      protein-1 (MCP-1) is a target to prevent from inflammation and atherogenic 
      changes in patient with diabetes mellitus. This study was made to determine 
      whether KLF5 may associate with MCP-1 expression in human umbilical vein 
      endothelial cells (HUVECs) induced by tumor necrosis factor-alpha (TNF-alpha), in 
      terms of the initial events of damaged vascular cells in diabetes. MCP-1 
      expression was markedly augmented by the treatment of TNF-alpha to HUVECs, but 
      this augmentation was inhibited by KLF5 small interfering RNA, which primarily 
      suppressed the expression of KLF5 at mRNA levels in the cells. Though TNF-alpha 
      augmented the levels of endothelin-1 (ET-1) and attenuated those of embryonic 
      form of myosin heavy chain (SMemb) in HUVECs, the inhibition of KLF5 did not 
      affect the levels of these cytokines in the cells. These results suggested that 
      in HUVECs, KLF5 is playing a critical role in regulating the expression of MCP-1, 
      which has been considered to be involved in the diabetic atherogenic events.
FAU - Kumekawa, Mari
AU  - Kumekawa M
AD  - Third Department of Internal Medicine, Tokyo Medical University, Tokyo, Japan.
FAU - Fukuda, Gen
AU  - Fukuda G
FAU - Shimizu, Saiko
AU  - Shimizu S
FAU - Konno, Kazushige
AU  - Konno K
FAU - Odawara, Masato
AU  - Odawara M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Endothelin-1)
RN  - 0 (KLF5 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Blotting, Western
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors
MH  - Culture Media, Conditioned
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Endothelin-1/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Kruppel-Like Transcription Factors/*genetics
MH  - RNA/biosynthesis/isolation & purification
MH  - RNA, Small Interfering/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/cytology/drug effects/metabolism
EDAT- 2008/08/02 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/08/02 09:00
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - JST.JSTAGE/bpb/31.1609 [pii]
AID - 10.1248/bpb.31.1609 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2008 Aug;31(8):1609-13. doi: 10.1248/bpb.31.1609.