PMID- 18670357
OWN - NLM
STAT- MEDLINE
DCOM- 20080923
LR  - 20220318
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 32
IP  - 9
DP  - 2008 Sep
TI  - DNA ploidy abnormalities in basal and superficial regions of the crypts in 
      Barrett's esophagus and associated neoplastic lesions.
PG  - 1327-35
LID - 10.1097/PAS.0b013e31816b6459 [doi]
AB  - The purpose of this study was to define the zonal DNA content distribution in the 
      basal versus the superficial crypt cells in Barrett's esophagus (BE) and related 
      neoplastic lesions. One hundred and five tissue sections of BE patients and 12 
      gastric tissue section as controls were stained with hematoxylin-eosin and 
      Feulgen and high-fidelity DNA histograms were generated from whole crypts (n=117) 
      and also separately from the basal and superficial portions of the crypts (n=71). 
      Three parameters were analyzed: (1) peak DNA index (DI), classified into diploidy 
      (DI=0.9-1.1) or aneuploidy (DI>1.1), the latter of which was further divided into 
      3 types: mild (DI=1.1-1.3), moderate (DI=1.3-1.8), and severe (DI>1.8). (2) 
      Heterogeneity index (HI), representing groups of cells with different DNA 
      content. (3) Percentage of cells with DI exceeding 5N rate (5N-ER). In full 
      crypts, compared with gastric controls, the prevalence of DNA aneuploidy 
      increased significantly (P<0.01) from nondysplastic BE to basal crypt dysplasia 
      (BCD), low-grade dysplasia (LGD), high-grade dysplasia (HGD), and adenocarcinoma 
      (AC). Nondysplastic BE, BCD, and LGD had mostly mild aneuploidy, and the majority 
      of HGD and AC had either moderate or severe aneuploidy. In addition, both HI and 
      5N-ER increased progressively from BCD and LGD to HGD and AC (P<0.01). When 
      analyzed separately, the superficial crypt cells were diploid in nondysplastic BE 
      and BCD, but were aneuploid in 50% of LGD and 100% of HGD cases. In contrast, 
      basal crypt cells were aneuploid in 37% of nondysplastic BE, 50% of BCD, 73% of 
      LGD, and 100% of HGD cases. A similar progressive increase in the HI and 5N-ER 
      values in basal crypt cells was observed with dysplastic progression. The changes 
      in DNA ploidy profiles of basal crypt cells in BCD and LGD were remarkably 
      similar. These results suggest that with neoplastic progression, dysplastic 
      changes in BE begin in the basal crypt cells and then extend further up the 
      crypts, and BCD represents a true early form of dysplasia limited to the crypt 
      bases.
FAU - Zhang, Xiaoqi
AU  - Zhang X
AD  - Departments of Medicine, Veterans Affairs Boston Healthcare System, Harvard 
      Medical School, West Roxbury, MA 02132, USA.
FAU - Huang, Qin
AU  - Huang Q
FAU - Goyal, Raj K
AU  - Goyal RK
FAU - Odze, Robert D
AU  - Odze RD
LA  - eng
GR  - DK62687/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Barrett Esophagus/*genetics
MH  - DNA/*analysis
MH  - Esophageal Neoplasms/*genetics
MH  - Humans
MH  - Image Cytometry
MH  - Image Processing, Computer-Assisted
MH  - *Ploidies
MH  - Precancerous Conditions/*genetics
EDAT- 2008/08/02 09:00
MHDA- 2008/09/24 09:00
CRDT- 2008/08/02 09:00
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2008/09/24 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - 10.1097/PAS.0b013e31816b6459 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2008 Sep;32(9):1327-35. doi: 10.1097/PAS.0b013e31816b6459.