PMID- 18670575
OWN - NLM
STAT- MEDLINE
DCOM- 20081014
LR  - 20190703
IS  - 1539-4522 (Electronic)
IS  - 1559-128X (Linking)
VI  - 47
IP  - 22
DP  - 2008 Aug 1
TI  - Simultaneous measurement of changes in light absorption due to the reduction of 
      cytochrome c oxidase and light scattering in rat brains during loss of tissue 
      viability.
PG  - 4164-76
AB  - We performed the simultaneous measurement of intrinsic optical signals (IOSs) 
      related to metabolic activity and cellular and subcellular morphological 
      characteristics, i.e., light scattering for a rat global ischemic brain model 
      made by rapidly removing blood by saline infusion. The signals were measured on 
      the basis of multiwavelength diffuse reflectances in which 605 and 830 nm were 
      used to detect the IOSs that are thought to be dominantly affected by redox 
      changes of heme aa(3) and CuA in cytochrome c oxidase (CcO), respectively. For 
      measuring the scattering signal, the wavelength that was found to be most 
      insensitive to the absorption changes, e.g., approximately 620 nm, was used. The 
      measurements suggested that an increase in the absorption due to reduction of 
      heme aa(3) occurred soon after blood clearance, and this was followed by a large 
      triphasic change in light scattering, during which time a decrease in the 
      absorption due to reduction of CuA occurred. Through the triphasic scattering 
      change, scattering signals increased by 5.2 +/- 1.5% (n = 5), and the increase in 
      light scattering showed significant correlation with both the reflectance 
      intensity changes at 605 and 830 nm. This suggests that morphological changes in 
      cells correlate with reductions of heme aa(3) and CuA. Histological analysis of 
      tissue after the triphasic scattering change showed no alteration in either the 
      nuclei or the cytoskeleton, but electron microscopic observation revealed 
      deformed, enlarged mitochondria and expanded dendrites. These findings suggest 
      that the simultaneous measurement of absorption signals related to the redox 
      changes in the CcO and the scattering signal is useful for monitoring tissue 
      viability in the brain.
FAU - Kawauchi, Satoko
AU  - Kawauchi S
AD  - Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, 
      Tokorozawa, Saitama 359-8513, Japan. skawauch@ndmc.ac.jp
FAU - Sato, Shunichi
AU  - Sato S
FAU - Ooigawa, Hidetoshi
AU  - Ooigawa H
FAU - Nawashiro, Hiroshi
AU  - Nawashiro H
FAU - Ishihara, Miya
AU  - Ishihara M
FAU - Kikuchi, Makoto
AU  - Kikuchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Opt
JT  - Applied optics
JID - 0247660
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Brain Diseases/*diagnosis/enzymology
MH  - Electron Transport Complex IV/*analysis
MH  - Light
MH  - Male
MH  - Oxidation-Reduction
MH  - Photometry/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Scattering, Radiation
MH  - Spectrum Analysis/*methods
EDAT- 2008/08/02 09:00
MHDA- 2008/10/15 09:00
CRDT- 2008/08/02 09:00
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2008/10/15 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - 170150 [pii]
AID - 10.1364/ao.47.004164 [doi]
PST - ppublish
SO  - Appl Opt. 2008 Aug 1;47(22):4164-76. doi: 10.1364/ao.47.004164.