PMID- 18672025
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20080916
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 444
IP  - 2
DP  - 2008 Oct 24
TI  - Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann 
      cells.
PG  - 143-7
LID - 10.1016/j.neulet.2008.07.055 [doi]
AB  - Anoikis is a type of apoptosis due to the detachment from the extracellular 
      matrix and neighboring cells. In case of cell transplantation therapy for spinal 
      cord injury, preparation of graft cells includes dissociation of cultured cells, 
      which may cause anoikis-induced cell death. Thus suppression of anoikis may 
      increase survival of grafted cells. Here we tested the effect of brain-derived 
      neurotrophic factor (BDNF) on anoikis-induced cell death of cultured Schwann 
      cells. Schwann cells were collected and cultured from sciatic nerves of neonatal 
      Wistar rats. Schwann cells were plated upon a non-adherent polyhydroxyethyl 
      methacrylate substrate to induce anoikis. BDNF was added into the culture medium 
      at various concentrations. Twenty-four hours after non-adherent culture, 
      approximately 40% of Schwann cells died and BDNF significantly decreased the 
      number of dead cells in that culture condition. Next, Schwann cells were 
      transplanted with or without BDNF treatment into contused rat spinal cord 1 week 
      after injury. Five weeks after transplantation, immunohistochemistry revealed 
      that the number of transplanted cells was significantly larger in the 
      BDNF-treated group than that of the non-treated group. Suppression of anoikis may 
      increase survival of grafted cells in case of cell therapy for spinal cord 
      injury.
FAU - Koda, Masao
AU  - Koda M
AD  - Department of Orthopaedic Surgery, Chiba Aoba Municipal Hospital, Chiba, Japan. 
      masaokoda@yahoo.co.jp
FAU - Someya, Yukio
AU  - Someya Y
FAU - Nishio, Yutaka
AU  - Nishio Y
FAU - Kadota, Ryo
AU  - Kadota R
FAU - Mannoji, Chikato
AU  - Mannoji C
FAU - Miyashita, Tomohiro
AU  - Miyashita T
FAU - Okawa, Akihiko
AU  - Okawa A
FAU - Murata, Atsushi
AU  - Murata A
FAU - Yamazaki, Masashi
AU  - Yamazaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080724
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Anoikis
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Female
MH  - Immunohistochemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Schwann Cells/*drug effects/physiology/transplantation
MH  - Sciatic Nerve/cytology
MH  - Spinal Cord Injuries/pathology/therapy
EDAT- 2008/08/02 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/08/02 09:00
PHST- 2007/05/03 00:00 [received]
PHST- 2008/06/22 00:00 [revised]
PHST- 2008/07/21 00:00 [accepted]
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - S0304-3940(08)01044-6 [pii]
AID - 10.1016/j.neulet.2008.07.055 [doi]
PST - ppublish
SO  - Neurosci Lett. 2008 Oct 24;444(2):143-7. doi: 10.1016/j.neulet.2008.07.055. Epub 
      2008 Jul 24.