PMID- 18672044
OWN - NLM
STAT- MEDLINE
DCOM- 20081113
LR  - 20111117
IS  - 0378-5955 (Print)
IS  - 0378-5955 (Linking)
VI  - 243
IP  - 1-2
DP  - 2008 Sep
TI  - Neurite outgrowth on cultured spiral ganglion neurons induced by erythropoietin.
PG  - 121-6
LID - 10.1016/j.heares.2008.07.003 [doi]
AB  - The morphological correlate of deafness is the loss of hair cells with subsequent 
      degeneration of spiral ganglion neurons (SGN). Neurotrophic factors have a 
      neuroprotective effect, and especially brain-derived neurotrophic factor (BDNF) 
      has been demonstrated to protect SGN in vitro and after ototoxic trauma in vivo. 
      Erythropoietin (EPO) attenuates hair cell loss in rat cochlea explants that were 
      treated with gentamycin. Recently, it has also been shown that EPO reduces the 
      apoptose rate in hippocampal neurons. Therefore, the aim of the study was to 
      examine the effects of EPO on SGN in vitro. Spiral ganglion cells were isolated 
      from neonatal rats and cultured for 48 h in serum-free medium supplemented with 
      EPO and/or BDNF. Results showed that survival rates of SGN were not significantly 
      improved when cultivated with EPO alone. Also, EPO did not further increase 
      BDNF-induced survival of SGN. However, significant elongation of neurites was 
      determined when SGN were cultivated with EPO alone. Even though a less than 
      additive effect was observed, combined treatment with BDNF and EPO led to a 
      significant elongation of neurites when compared to individual treatment with 
      BDNF or EPO. It can be concluded that EPO induces neurite outgrowth rather than 
      promoting survival. Thus, EPO presents as an interesting candidate to enhance and 
      modulate the regenerative effect of BDNF on SGN.
FAU - Berkingali, Nurdanat
AU  - Berkingali N
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of 
      Hannover, Hannover, Germany.
FAU - Warnecke, Athanasia
AU  - Warnecke A
FAU - Gomes, Priya
AU  - Gomes P
FAU - Paasche, Gerrit
AU  - Paasche G
FAU - Tack, Jan
AU  - Tack J
FAU - Lenarz, Thomas
AU  - Lenarz T
FAU - Stover, Timo
AU  - Stover T
LA  - eng
PT  - Journal Article
DEP - 20080715
PL  - Netherlands
TA  - Hear Res
JT  - Hearing research
JID - 7900445
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Receptors, Erythropoietin)
RN  - 0 (Recombinant Proteins)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Erythropoietin/*pharmacology
MH  - Immunohistochemistry
MH  - Nerve Regeneration/drug effects
MH  - Neurites/*drug effects/*ultrastructure
MH  - Neurons/drug effects/metabolism/ultrastructure
MH  - Neuroprotective Agents/pharmacology
MH  - Rats
MH  - Receptors, Erythropoietin/metabolism
MH  - Recombinant Proteins
MH  - Spiral Ganglion/*drug effects/metabolism/*ultrastructure
EDAT- 2008/08/02 09:00
MHDA- 2008/11/14 09:00
CRDT- 2008/08/02 09:00
PHST- 2007/08/02 00:00 [received]
PHST- 2008/06/27 00:00 [revised]
PHST- 2008/07/02 00:00 [accepted]
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2008/11/14 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - S0378-5955(08)00131-7 [pii]
AID - 10.1016/j.heares.2008.07.003 [doi]
PST - ppublish
SO  - Hear Res. 2008 Sep;243(1-2):121-6. doi: 10.1016/j.heares.2008.07.003. Epub 2008 
      Jul 15.