PMID- 18672339
OWN - NLM
STAT- MEDLINE
DCOM- 20090320
LR  - 20131121
IS  - 0399-077X (Print)
IS  - 0399-077X (Linking)
VI  - 38
IP  - 10
DP  - 2008 Oct
TI  - Genotypic screening of atypical Staphylococcus aureus strains isolated from 
      clinical samples for presence of selected adhesin genes.
PG  - 549-53
LID - 10.1016/j.medmal.2008.06.003 [doi]
AB  - OBJECTIVE: The aim of this study was to screen Staphylococcus aureus negative for 
      production of coagulase or clumping factor and for presence of selected adhesin 
      genes. METHODS: Sixty coagulase-negative and 20 clumping factor-negative S. 
      aureus strains were studied. Detection of methicillin resistance was performed 
      using the agar screen technique with 6 mg/L of oxacillin and was confirmed by 
      amplification mecA gene. The presence of bone binding protein (bbp), collagen 
      binding protein (cna), fibronectin A binding protein (fnbA), fibronectin B 
      binding protein (fnbB) and clumping factor A (clfA) genes was detected by 
      multiplex PCR. RESULTS: Almost all (98%) of the strains were positive for clfA 
      gene. There were fnbA and fnibB in 85%, cna in 54% and bbp in 5% of strains 
      found. No correlation between presence of the particular genes and clinical 
      samples was observed. The prevalence of fnbA, fnbB and cna was statistically 
      higher in coagulase-negative than in clumping factor-negative strains (89, 89, 66 
      and 70, 70, 15%, respectively). Similarly, all of these genes were more often 
      observed in MRSA than in MSSA atypical strains. The cna was detected only in 
      coagulase-negative MRSA.
FAU - Wisniewska, K
AU  - Wisniewska K
AD  - Department of Medical Microbiology, Medical University of Gdansk, 38, Do 
      Studzienki Street, 80-227 Gdansk, Poland. kwis@amg.gda.pl
FAU - Garbacz, K
AU  - Garbacz K
FAU - Piechowicz, L
AU  - Piechowicz L
LA  - eng
PT  - Journal Article
PL  - France
TA  - Med Mal Infect
JT  - Medecine et maladies infectieuses
JID - 0311416
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Coagulase)
RN  - 0 (adhesin, Staphylococcus aureus)
RN  - 0 (bone sialoprotein-binding protein, Staphylococcus aureus)
RN  - 0 (fibronectin-binding proteins, bacterial)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Adhesins, Bacterial/*genetics
MH  - Bacterial Proteins/genetics
MH  - Bacterial Typing Techniques/*methods
MH  - Carrier Proteins/genetics
MH  - Coagulase/genetics
MH  - Drug Resistance, Multiple, Bacterial
MH  - *Genes, Bacterial
MH  - Humans
MH  - Methicillin Resistance
MH  - Methicillin-Resistant Staphylococcus aureus/genetics/isolation & purification
MH  - Oxacillin/pharmacology
MH  - Poland/epidemiology
MH  - Staphylococcal Infections/epidemiology/*microbiology
MH  - Staphylococcus aureus/classification/drug effects/*genetics/isolation & 
      purification
EDAT- 2008/08/02 09:00
MHDA- 2009/03/21 09:00
CRDT- 2008/08/02 09:00
PHST- 2007/06/04 00:00 [received]
PHST- 2008/06/03 00:00 [accepted]
PHST- 2008/08/02 09:00 [pubmed]
PHST- 2009/03/21 09:00 [medline]
PHST- 2008/08/02 09:00 [entrez]
AID - S0399-077X(08)00159-5 [pii]
AID - 10.1016/j.medmal.2008.06.003 [doi]
PST - ppublish
SO  - Med Mal Infect. 2008 Oct;38(10):549-53. doi: 10.1016/j.medmal.2008.06.003.