PMID- 18674523
OWN - NLM
STAT- MEDLINE
DCOM- 20081208
LR  - 20161124
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1232
DP  - 2008 Sep 26
TI  - Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after 
      post-natal nicotine and intermittent hypercapnic hypoxia.
PG  - 195-205
LID - 10.1016/j.brainres.2008.07.039 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) and its receptor TrkB play a significant 
      role in the regulation of cell growth, survival and death during central nervous 
      system development. The expression of BDNF and TrkB is affected by noxious 
      insults. Two insults during the early post-natal period that are of interest to 
      our laboratory are exposure to nicotine and to intermittent hypercapnic hypoxia 
      (IHH). Piglet models were used to mimic the conditions associated with the risk 
      factors for the sudden infant death syndrome (SIDS) including post-natal 
      cigarette smoke exposure (nicotine model) and prone sleeping where the infant is 
      subjected to re-breathing of expired gases (IHH model). We aimed to determine the 
      effects of nicotine and IHH, alone or in combination, on pro- and rhBDNF and TrkB 
      expression in the developing piglet brainstem. Four piglet groups were studied, 
      with equal gender ratios in each: control (n=14), nicotine (n=14), IHH (n=10) and 
      nic+IHH (n=14). Applying immunohistochemistry, and studying six nuclei of the 
      caudal medulla, we found that compared to controls, TrkB was the only protein 
      significantly decreased after nicotine and nic+IHH exposure regardless of gender. 
      For pro-BDNF and rhBDNF however, observed changes were more evident in males than 
      females exposed to nicotine and nic+IHH. The implications of these findings are 
      that a prior nicotine exposure makes the developing brainstem susceptible to 
      greater changes in the neurotrophic effects of BDNF and its receptor TrkB in the 
      face of a hypoxic insult, and that the effects are greater in males than females.
FAU - Tang, Samantha
AU  - Tang S
AD  - Department of Paediatrics and Child Health, The University of Sydney, and The 
      Children's Hospital, Westmead Sydney, NSW 2006, Australia.
FAU - Machaalani, Rita
AU  - Machaalani R
FAU - Waters, Karen A
AU  - Waters KA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080719
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Recombinant Proteins)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis/drug effects
MH  - Brain Stem/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Survival/drug effects
MH  - Female
MH  - Hypercapnia/*psychology
MH  - Hypoxia/*psychology
MH  - Immunohistochemistry
MH  - Male
MH  - Medulla Oblongata/drug effects/growth & development
MH  - Nicotine/*pharmacology
MH  - Nicotinic Agonists/*pharmacology
MH  - Receptor, trkB/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sex Characteristics
MH  - Swine
EDAT- 2008/08/05 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/05 09:00
PHST- 2008/04/15 00:00 [received]
PHST- 2008/07/03 00:00 [revised]
PHST- 2008/07/07 00:00 [accepted]
PHST- 2008/08/05 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/05 09:00 [entrez]
AID - S0006-8993(08)01679-X [pii]
AID - 10.1016/j.brainres.2008.07.039 [doi]
PST - ppublish
SO  - Brain Res. 2008 Sep 26;1232:195-205. doi: 10.1016/j.brainres.2008.07.039. Epub 
      2008 Jul 19.