PMID- 18675423
OWN - NLM
STAT- MEDLINE
DCOM- 20081015
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1203
IP  - 2
DP  - 2008 Sep 5
TI  - Multi-residue determination of seven quinolones antibiotics in gilthead seabream 
      using liquid chromatography-tandem mass spectrometry.
PG  - 115-23
LID - 10.1016/j.chroma.2008.07.003 [doi]
AB  - A sensitive and selective confirmatory analytical method for the multi-residue 
      determination of seven quinolones (ciprofloxacin, enrofloxacin, sarafloxacin, 
      danofloxacin, oxolinic acid, nalidixic acid and flumequine) in gilthead seabream 
      (Sparus aurata) was developed. The sample pre-treatment involves extraction with 
      0.1 M NaOH and purification by solid-phase extraction (SPE) on Waters Oasis HLB 
      cartridges followed by the determination of all compounds in a single 
      LC-electrospray ionization MS/MS run. Separation was achieved on a Perfectsil 
      ODS-2, 5 microm, 250 mm x 4 mm, analytical column (MZ Analysentechnik) by 
      gradient elution using a mixture of 0.2% (v/v) formic acid, methanol and 
      acetonitrile within 30 min. Multiple reaction monitoring (MRM) was used for 
      selective detection of each quinolone. Accuracy was evaluated through recovery 
      studies at three different fortification levels. The mean recoveries are between 
      90 and 132% for the selected levels with RSD values lower than 20%. The method 
      presents satisfactory results for linearity, precision and limits of 
      quantification. The latter are much lower than the maximum residue limits (MRLs) 
      established by the European Union for quinolones in fish tissues (6-8 microg/kg).
FAU - Samanidou, Victoria
AU  - Samanidou V
AD  - Aristotle University of Thessaloniki, Laboratory of Analytical Chemistry, 
      GR-54124 Thessaloniki, Greece. samanidu@chem.auth.gr
FAU - Evaggelopoulou, Evaggelia
AU  - Evaggelopoulou E
FAU - Trotzmuller, Martin
AU  - Trotzmuller M
FAU - Guo, Xinghua
AU  - Guo X
FAU - Lankmayr, Ernst
AU  - Lankmayr E
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20080709
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Quinolones)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Aquaculture/methods
MH  - Chromatography, Liquid/*methods
MH  - Fish Products/*analysis
MH  - Quinolones/*analysis
MH  - Reproducibility of Results
MH  - Sea Bream
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2008/08/05 09:00
MHDA- 2008/10/16 09:00
CRDT- 2008/08/05 09:00
PHST- 2008/04/09 00:00 [received]
PHST- 2008/06/26 00:00 [revised]
PHST- 2008/07/02 00:00 [accepted]
PHST- 2008/08/05 09:00 [pubmed]
PHST- 2008/10/16 09:00 [medline]
PHST- 2008/08/05 09:00 [entrez]
AID - S0021-9673(08)01131-X [pii]
AID - 10.1016/j.chroma.2008.07.003 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Sep 5;1203(2):115-23. doi: 10.1016/j.chroma.2008.07.003. 
      Epub 2008 Jul 9.