PMID- 18677504
OWN - NLM
STAT- MEDLINE
DCOM- 20081216
LR  - 20211020
IS  - 1439-6319 (Print)
IS  - 1439-6319 (Linking)
VI  - 104
IP  - 5
DP  - 2008 Nov
TI  - Long-term resistance training improves force and unloaded shortening velocity of 
      single muscle fibres of elderly women.
PG  - 885-93
LID - 10.1007/s00421-008-0845-0 [doi]
AB  - Four elderly women (78 +/- 4.3 years) were resistance trained (RT) for one year 
      and needle biopsies of the vastus lateralis muscle were taken pre- and post-RT. A 
      training intensity of 60% one-repetition maximum (1RM) was adopted for the entire 
      duration of the study. Following RT, significant gain in isometric force of the 
      quadriceps muscles was observed and MHC isoform distribution of vastus lateralis 
      muscle showed a very significant shift from MHC-1 and MHC-2X towards MHC-2A, i.e. 
      a bi-directional shift. A large population (n = 310) of individual skinned muscle 
      fibres were dissected from biopsy samples and used for determination of 
      cross-sectional area (CSA), specific force (Po/CSA) and unloaded shortening 
      velocity (Vo) at 12 degrees C and maximum activation. Fibres were classified on 
      the basis of their MHC isoform content determined by SDS-PAGE in three pure fibre 
      types (1, 2A and 2X) and two hybrid fibre types (1-2A and 2AX). Whereas the CSA 
      of individual muscle fibres did not change, Po/CSA was significantly higher in 
      both type 1 and type 2A fibres post training. Vo of type 1 fibres was 
      significantly higher post-training, whereas no change in Vo was observed in type 
      2A fibres. The number of pure type 2X fibres was very low especially 
      post-training and did not enable a statistically significant comparison. The data 
      suggest that in elderly women moderate and prolonged RT: (1) can determine 
      similar adaptations in MHC distribution of skeletal muscle to those expected in 
      young subjects; (2) can improve function of muscle fibres.
FAU - Parente, Valeria
AU  - Parente V
AD  - Department of Experimental Medicine, Human Physiology unit, and Interuniversity 
      Institute of Miology, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy.
FAU - D'Antona, Giuseppe
AU  - D'Antona G
FAU - Adami, Raffaella
AU  - Adami R
FAU - Miotti, Danilo
AU  - Miotti D
FAU - Capodaglio, Paolo
AU  - Capodaglio P
FAU - De Vito, Giuseppe
AU  - De Vito G
FAU - Bottinelli, Roberto
AU  - Bottinelli R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080802
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (Protein Isoforms)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Adaptation, Physiological
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Humans
MH  - *Muscle Contraction
MH  - Muscle Fibers, Skeletal/metabolism/*physiology
MH  - *Muscle Strength
MH  - Myosin Heavy Chains/metabolism
MH  - Protein Isoforms
MH  - Quadriceps Muscle/cytology/metabolism/*physiology
MH  - *Resistance Training
EDAT- 2008/08/05 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/05 09:00
PHST- 2008/07/23 00:00 [accepted]
PHST- 2008/08/05 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/05 09:00 [entrez]
AID - 10.1007/s00421-008-0845-0 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2008 Nov;104(5):885-93. doi: 10.1007/s00421-008-0845-0. Epub 
      2008 Aug 2.