PMID- 1867862
OWN - NLM
STAT- MEDLINE
DCOM- 19910916
LR  - 20051117
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 10
IP  - 4
DP  - 1991 Apr
TI  - A new cationic liposome reagent mediating nearly quantitative transfection of 
      animal cells.
PG  - 520-5
AB  - One of the most efficient systems for the expression of genes in the cytoplasm of 
      animal cells utilizes a recombinant vaccinia virus encoding the bacteriophage T7 
      RNA polymerase. Cells infected with this virus are transfected with plasmid DNAs 
      containing the gene to be expressed under T7 promoter control. The major 
      limitation of this system is the efficiency with which DNA is introduced into the 
      cell. Recently, a cationic liposome-mediated transfection reagent has yielded 
      transfection frequencies of greater than 80%. To determine if commercially 
      available cationic lipids could form liposomes that would yield similar 
      transfection efficiencies, we tested liposomes prepared with five different 
      cationic lipids. When used at appropriate concentrations in liposomes that also 
      contained a neutral lipid, four of the five cationic lipids were effective in the 
      transfection of HeLa cells. However, liposomes formed with the neutral lipid and 
      one of the cationic lipids, dimethyldioctadecylammonium bromide (DDAB), gave 
      transfection frequencies of greater than 95% and had a broad spectrum of 
      effectiveness on a variety of cell lines. Liposomes containing DDAB are an 
      inexpensive, highly efficient and reproducible alternative for the transfection 
      of animal cells and are well suited for use with the vaccinia virus/T7 expression 
      system.
FAU - Rose, J K
AU  - Rose JK
AD  - Yale University School of Medicine.
FAU - Buonocore, L
AU  - Buonocore L
FAU - Whitt, M A
AU  - Whitt MA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (Cations)
RN  - 0 (Liposomes)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 13146-86-6 (didodecyldimethylammonium)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cations
MH  - Cell Line
MH  - DNA
MH  - Dogs
MH  - Flow Cytometry
MH  - HeLa Cells
MH  - Humans
MH  - *Liposomes
MH  - Microscopy, Fluorescence
MH  - Quaternary Ammonium Compounds
MH  - *Transfection
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PST - ppublish
SO  - Biotechniques. 1991 Apr;10(4):520-5.