PMID- 18678936
OWN - NLM
STAT- MEDLINE
DCOM- 20080821
LR  - 20240415
IS  - 1744-3091 (Electronic)
IS  - 1744-3091 (Linking)
VI  - 64
IP  - Pt 8
DP  - 2008 Aug 1
TI  - Crystallization and preliminary X-ray analysis of the inducible lysine 
      decarboxylase from Escherichia coli.
PG  - 700-6
LID - 10.1107/S1744309108018757 [doi]
AB  - The decameric inducible lysine decarboxylase (LdcI) from Escherichia coli has 
      been crystallized in space groups C2 and C222(1); the Ta6Br12(2+) cluster was 
      used to derivatize the C2 crystals. The method of single isomorphous replacement 
      with anomalous scattering (SIRAS) as implemented in SHELXD was used to solve the 
      Ta6Br12(2+)-derivatized structure to 5 A resolution. Many of the 
      Ta6Br12(2+)-binding sites had twofold and fivefold noncrystallographic symmetry. 
      Taking advantage of this feature, phase modification was performed in DM. The 
      electron-density map of LdcI displays many features in agreement with the 
      low-resolution negative-stain electron-density map [Snider et al. (2006), J. 
      Biol. Chem. 281, 1532-1546].
FAU - Alexopoulos, Eftichia
AU  - Alexopoulos E
AD  - Department of Biochemistry, University of Toronto, 1 King's College Circle, 
      Medical Sciences Building, Toronto, Ontario M5S1A8, Canada.
FAU - Kanjee, Usheer
AU  - Kanjee U
FAU - Snider, Jamie
AU  - Snider J
FAU - Houry, Walid A
AU  - Houry WA
FAU - Pai, Emil F
AU  - Pai EF
LA  - eng
PT  - Journal Article
DEP - 20080705
PL  - England
TA  - Acta Crystallogr Sect F Struct Biol Cryst Commun
JT  - Acta crystallographica. Section F, Structural biology and crystallization 
      communications
JID - 101226117
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.18 (lysine decarboxylase)
SB  - IM
MH  - Carboxy-Lyases/*chemistry/genetics/isolation & purification
MH  - Chromatography, Gel
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Escherichia coli/*enzymology
MH  - Protein Conformation
PMC - PMC2494963
EDAT- 2008/08/06 09:00
MHDA- 2008/08/22 09:00
PMCR- 2009/08/01
CRDT- 2008/08/06 09:00
PHST- 2008/05/12 00:00 [received]
PHST- 2008/06/20 00:00 [accepted]
PHST- 2008/08/06 09:00 [pubmed]
PHST- 2008/08/22 09:00 [medline]
PHST- 2008/08/06 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - S1744309108018757 [pii]
AID - en5307 [pii]
AID - 10.1107/S1744309108018757 [doi]
PST - ppublish
SO  - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):700-6. doi: 
      10.1107/S1744309108018757. Epub 2008 Jul 5.