PMID- 18680554
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20161124
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 107
IP  - 1
DP  - 2008 Oct
TI  - Lysophosphatidic acid-induced membrane ruffling and brain-derived neurotrophic 
      factor gene expression are mediated by ATP release in primary microglia.
PG  - 152-60
LID - 10.1111/j.1471-4159.2008.05599.x [doi]
AB  - We examined the effects of lysophosphatidic acid (LPA) on microglia, which may 
      play an important role in the development and maintenance of neuropathic pain. 
      LPA caused membrane ruffling as detected by scanning electron microscopy, and 
      increased the expression of brain-derived neurotrophic factor (BDNF) in a primary 
      culture of rat microglia, which express LPA(3), but not LPA(1) or LPA(2) 
      receptors. These actions were inhibited by a Galpha(q/11)-antisense 
      oligodeoxynucleotide (AS-ODN), U73122, an inhibitor of phospholipase C (PLC), and 
      apyrase, which specifically degrades ATP and ADP. When ATP release was measured 
      using a luciferin-luciferase bioluminescence assay, LPA was shown to increase it 
      in an LPA(3) and PLC inhibitor-reversible manner. However, LPA-induced ATP 
      release was also blocked by the Galpha(q/11) AS-ODN, but not by pertussis toxin. 
      These results suggest that LPA induces the release of ATP from rat primary 
      cultured microglia via the LPA(3) receptor, Galpha(q/11) and PLC, and that the 
      released ATP or ectopically converted ADP may in turn cause membrane ruffling via 
      P2Y(12) receptors and Galpha(i/o) activation, and BDNF expression via activation 
      of P2X(4) receptors.
FAU - Fujita, Ryousuke
AU  - Fujita R
AD  - Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate 
      School of Biomedical Sciences, Bunkyo-machi, Nagasaki, Japan.
FAU - Ma, Yan
AU  - Ma Y
FAU - Ueda, Hiroshi
AU  - Ueda H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080731
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Lysophospholipids)
RN  - 0 (P2rx4 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Lysophosphatidic Acid)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2X4)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gq-G11)
RN  - PG6M3969SG (lysophosphatidic acid)
SB  - IM
MH  - Adenosine Diphosphate/metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Brain/*metabolism/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Surface Extensions/drug effects/*metabolism/ultrastructure
MH  - Cells, Cultured
MH  - GTP-Binding Protein alpha Subunits, Gq-G11/antagonists & 
      inhibitors/genetics/metabolism
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Lysophospholipids/*pharmacology
MH  - Microglia/drug effects/*metabolism/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Lysophosphatidic Acid/drug effects/metabolism
MH  - Receptors, Purinergic P2/drug effects/metabolism
MH  - Receptors, Purinergic P2X4
MH  - Type C Phospholipases/antagonists & inhibitors/metabolism
EDAT- 2008/08/06 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/08/06 09:00
PHST- 2008/08/06 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/08/06 09:00 [entrez]
AID - JNC5599 [pii]
AID - 10.1111/j.1471-4159.2008.05599.x [doi]
PST - ppublish
SO  - J Neurochem. 2008 Oct;107(1):152-60. doi: 10.1111/j.1471-4159.2008.05599.x. Epub 
      2008 Jul 31.