PMID- 18683135
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20221207
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 25
IP  - 4
DP  - 2008 Aug
TI  - [Sequence analysis of a novel human leukocyte antigen allele HLA-A*9206].
PG  - 396-9
AB  - OBJECTIVE: To investigate the molecular genetic basis for a human leukocyte 
      antigen (HLA) novel allele HLA-A*9206 in the Chinese population. METHODS: DNA was 
      extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of 
      HLA-A exons 1-8 of the proband was preformed and the PCR products were sequenced 
      using ABI sequencing kit. Both strands of exons 2, 3 and 4 of the amplified 
      product were sequenced. The polymerase chain reaction-sequence specific primer 
      (PCR-SSP) was performed to split the two alleles apart and confirm the mutations 
      detected by sequencing. RESULTS: The sequencing results showed that the HLA-A 
      alleles of the proband were A*1101 and a novel allele. The sequence of the novel 
      allele has been submitted to GenBank (EF062306). After Blast analysis, the novel 
      allele shows one nucleotide different from the HLA-A*0206 in exon 3 at nucleotide 
      position 530 (C to T). This results in an amino acid change from Ala to Val at 
      codon 153. CONCLUSION: This allele is a novel allele and has been officially 
      named A*9206 by the WHO Nomenclature Committee.
FAU - He, Jun-jun
AU  - He JJ
AD  - Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of 
      Ministry of Health, Hangzhou, Zhejiang, 310006 People's Republic of China.
FAU - Zhang, Wei
AU  - Zhang W
FAU - Wang, Wei
AU  - Wang W
FAU - Han, Zhe-dong
AU  - Han ZD
FAU - Chen, Nan-ying
AU  - Chen NY
FAU - Zhu, Fa-ming
AU  - Zhu FM
FAU - Yan, Li-xing
AU  - Yan LX
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (HLA-A Antigens)
SB  - IM
MH  - Alleles
MH  - Asian People
MH  - Base Sequence
MH  - HLA-A Antigens/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 2008/08/07 09:00
MHDA- 2009/04/03 09:00
CRDT- 2008/08/07 09:00
PHST- 2008/08/07 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
PHST- 2008/08/07 09:00 [entrez]
AID - 940625085 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Aug;25(4):396-9.