PMID- 18683215
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20230202
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 114
IP  - 5
DP  - 2008 Oct 25
TI  - Chromosomal abnormalities detected by multicolor fluorescence in situ 
      hybridization in fine-needle aspirates from patients with small lymphocytic 
      lymphoma are useful for predicting survival.
PG  - 315-22
LID - 10.1002/cncr.23796 [doi]
AB  - BACKGROUND: Fine-needle aspiration (FNA) of lymph nodes is commonly used to 
      assess disease progression in patients with small lymphocytic lymphoma (SLL). 
      Although cytologic features are helpful for diagnosing typical SLL and 
      transformed large-cell lymphoma (tLCL), SLL in accelerated phase (SLLacc) is more 
      difficult to diagnose. Additional tests are needed to identify those patients who 
      are transforming to a higher-grade lymphoma. This study evaluated the use of a 
      multicolor fluorescence in situ hybridization (FISH) probe panel specifically 
      designed for chronic lymphocytic leukemia (CLL)/SLL and assessed the association 
      between FISH findings and cytologic diagnosis, proliferation index, and risk of 
      death. METHODS: FNA specimens from 50 patients (32 men and 18 women; mean age, 57 
      years [range, 36-77 years]) with histologically confirmed CLL and/or SLL were 
      evaluated in this study for chromosomal abnormalities of 11q22 (ATM), 12, 
      13q14.3, 13q34.3 (LAMP1), and 17p13.1 (p53) by using a multiprobe FISH kit. One 
      of the 50 cases was excluded because of an insufficient number of cells for FISH 
      analysis. The FISH findings were compared with the cytologic diagnoses (26 SLLs, 
      12 SLLaccs, and 11 tLCLs), Ki-67 immunostaining, and risk of death. RESULTS: 
      Abnormal signal patterns for 17p13.1 and 13q34.3 were associated with tLCL. 
      Aberrations of 17p13.1 were found to be significantly associated with Ki-67 
      staining. Of the 49 patients with interpretable FISH results, 22 (45%) had died 
      at the time of the study, with a mean overall survival time of 17 months after 
      FNA. Patients with aberrations of 17p13.1 and 11q22 had 3.7 and 2.7 times the 
      risk of death, respectively, compared with patients with normal patterns. 
      CONCLUSIONS: FISH can be performed on FNA specimens from patients with a history 
      of SLL/CLL. Chromosomal aberrations of 17p13.1 and 11q22 are associated with an 
      increased risk of death. Knowledge of genetic abnormalities from FNAs may be 
      useful in deciding when and how to treat indolent or progressive SLL.
CI  - (c) 2008 American Cancer Society.
FAU - Caraway, Nancy P
AU  - Caraway NP
AD  - Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 
      Houston, Texas 77030, USA. ncaraway@mdanderson.org
FAU - Thomas, Elizabeth
AU  - Thomas E
FAU - Khanna, Abha
AU  - Khanna A
FAU - Payne, Linda
AU  - Payne L
FAU - Zhang, Hua-Zhong
AU  - Zhang HZ
FAU - Lin, E
AU  - Lin E
FAU - Keating, Michael J
AU  - Keating MJ
FAU - Katz, Ruth L
AU  - Katz RL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Biopsy, Fine-Needle
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*mortality/surgery
MH  - Male
MH  - Middle Aged
EDAT- 2008/08/07 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/08/07 09:00
PHST- 2008/08/07 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/08/07 09:00 [entrez]
AID - 10.1002/cncr.23796 [doi]
PST - ppublish
SO  - Cancer. 2008 Oct 25;114(5):315-22. doi: 10.1002/cncr.23796.