PMID- 18687170
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20131121
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 20
IP  - 8
DP  - 2008 Aug
TI  - [The different effects of glutamine on macrophage cytokines release in vivo and 
      in vitro].
PG  - 456-60
AB  - OBJECTIVE: To evaluate the effects of glutamine (Gln) on macrophages cytokines 
      release and heat shock protein 72 (HSP72) expression in vivo and in vitro, and 
      explore the anti-inflammation mechanisms of Gln during sepsis. METHODS: In 
      experiment one, mouse peritoneal macrophage cell line RAW264.7 cells were divided 
      into 0, 0.5 and 8 mmol/L Gln groups, and cells and supernatants were harvested at 
      0, 1, 4, 12 and 24 hours after lipopolysaccharide (LPS) challenge. In experiment 
      two, forty-five Kunming mice were randomized into sham-operation group (Sham), 
      sepsis model group, and Gln group. Sepsis was induced by cecal ligation and 
      puncture (CLP). Either Gln 0.75 g/kg (Gln group) or saline (Sham and model group) 
      was administered immediately after CLP via tail-vein injection. Blood sample was 
      collected at 6 hours, and macrophages were harvested by peritoneal lavage. Tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-10 contents in 
      supernatant, serum and cell lysate were analyzed with enzyme-linked immunosorbent 
      assay (ELISA), macrophages HSP72 expression was assessed with Western blotting. 
      RESULTS: Gln promoted RAW264.7 cells to release TNF-alpha, IL-6 and IL-10 in a 
      dose-dependent and time-dependent manner in vitro (P<0.05 or P<0.01), and 
      significantly increased HSP72 expression in 8 mmol/L Gln group at 4 hours after 
      LPS stimulation (both P<0.01). In vivo, in animals given Gln intracellular 
      TNF-alpha and IL-6 levels were significantly lower than sepsis animals (P<0.01 
      and P<0.05), but there was no statistically significant difference in 
      intracellular IL-10 levels among three groups. The serum levels of TNF-alpha in 
      Gln group were significantly lower than in model group (P<0.05), while serum IL-6 
      and IL-10 levels were similar between two groups. Gln treatment led to 
      significant HSP72 expression compared to model and Sham groups (both P<0.01). 
      CONCLUSION: Gln can promote inflammatory cytokines release from macrophages in 
      vitro, which cannot be attenuated by HSP72 expression induced by Gln in LPS 
      challenged RAW264.7 macrophages. Gln treatment significantly decreases 
      intracellular TNF-alpha and IL-6 levels in vivo during sepsis. HSP72 expression 
      increases after Gln treatment both in vivo and in vitro. These data implicate 
      that HSP72 may not play a major role in attenuating the inflammatory response 
      after Gln administration in sepsis.
FAU - Wang, Xue-min
AU  - Wang XM
AD  - Department of Anesthesiology/Intensive Care Unit, Shanghai 6 th People's 
      Hospital, Shanghai Jiaotong University, Shanghai 200233, China. 
      wangxm@sjtu.edu.cn
FAU - Liang, Meng-fan
AU  - Liang MF
FAU - Yuan, Yuan
AU  - Yuan Y
FAU - Jiang, Wei
AU  - Jiang W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Cytokines)
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0RH81L854J (Glutamine)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Glutamine/*pharmacology
MH  - HSP72 Heat-Shock Proteins/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Peritoneal/drug effects/*metabolism
MH  - Mice
MH  - Random Allocation
MH  - Sepsis/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/08/09 09:00
MHDA- 2010/02/03 06:00
CRDT- 2008/08/09 09:00
PHST- 2008/08/09 09:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
PHST- 2008/08/09 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008 Aug;20(8):456-60.