PMID- 18687794
OWN - NLM
STAT- MEDLINE
DCOM- 20081016
LR  - 20211020
IS  - 1525-1578 (Print)
IS  - 1943-7811 (Electronic)
IS  - 1525-1578 (Linking)
VI  - 10
IP  - 5
DP  - 2008 Sep
TI  - Whole-genome scanning by array comparative genomic hybridization as a clinical 
      tool for risk assessment in chronic lymphocytic leukemia.
PG  - 442-51
LID - 10.2353/jmoldx.2008.080033 [doi]
AB  - Array-based comparative genomic hybridization (array CGH) provides a powerful 
      method for simultaneous genome-wide scanning and prognostic marker assessment in 
      chronic lymphocytic leukemia (CLL). In the current study, commercially available 
      bacterial artificial chromosome and oligonucleotide array CGH platforms were used 
      to identify chromosomal alterations of prognostic significance in 174 CLL cases. 
      Tumor genomes were initially analyzed by bacterial artificial chromosome array 
      CGH followed by confirmation and breakpoint mapping using oligonucleotide arrays. 
      Genomic changes involving loci currently interrogated by fluorescence in situ 
      hybridization (FISH) panels were detected in 155 cases (89%) at expected 
      frequencies: 13q14 loss (47%), trisomy 12 (13%), 11q loss (11%), 6q loss (7.5%), 
      and 17p loss (4.6%). Genomic instability was the second most commonly identified 
      alteration of prognostic significance with three or more alterations involving 
      loci not interrogated by FISH panels identified in 37 CLL cases (21%). A subset 
      of 48 CLL cases analyzed by six-probe FISH panels (288 total hybridizations) was 
      concordant with array CGH results for 275 hybridizations (95.5%); 13 
      hybridizations (4.5%) were discordant because of clonal populations that 
      comprised less than 30% of the sample. Array CGH is a powerful, cost-effective 
      tool for genome-wide risk assessment in the clinical evaluation of CLL.
FAU - Gunn, Shelly R
AU  - Gunn SR
AD  - The University of Texas Health Science Center at San Antonio, Department of 
      Pathology, Mail Code 7750, 7703 Floyd Curl Dr., San Antonio, TX, USA. 
      gunn@uthscsa.edu
FAU - Mohammed, Mansoor S
AU  - Mohammed MS
FAU - Gorre, Mercedes E
AU  - Gorre ME
FAU - Cotter, Philip D
AU  - Cotter PD
FAU - Kim, Jaeweon
AU  - Kim J
FAU - Bahler, David W
AU  - Bahler DW
FAU - Preobrazhensky, Sergey N
AU  - Preobrazhensky SN
FAU - Higgins, Russell A
AU  - Higgins RA
FAU - Bolla, Aswani R
AU  - Bolla AR
FAU - Ismail, Sahar H
AU  - Ismail SH
FAU - de Jong, Daphne
AU  - de Jong D
FAU - Eldering, Eric
AU  - Eldering E
FAU - van Oers, Marinus H J
AU  - van Oers MH
FAU - Mellink, Clemens H M
AU  - Mellink CH
FAU - Keating, Michael J
AU  - Keating MJ
FAU - Schlette, Ellen J
AU  - Schlette EJ
FAU - Abruzzo, Lynne V
AU  - Abruzzo LV
FAU - Robetorye, Ryan S
AU  - Robetorye RS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20080807
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
SB  - IM
MH  - Algorithms
MH  - Chromosome Breakage
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Genome, Human
MH  - Genomic Instability
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/*genetics
MH  - Molecular Diagnostic Techniques/*methods
MH  - Nucleic Acid Hybridization/*methods
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Risk Assessment
PMC - PMC2518739
EDAT- 2008/08/09 09:00
MHDA- 2008/10/17 09:00
PMCR- 2009/09/01
CRDT- 2008/08/09 09:00
PHST- 2008/08/09 09:00 [pubmed]
PHST- 2008/10/17 09:00 [medline]
PHST- 2008/08/09 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - S1525-1578(10)60182-1 [pii]
AID - JMDI60182 [pii]
AID - 10.2353/jmoldx.2008.080033 [doi]
PST - ppublish
SO  - J Mol Diagn. 2008 Sep;10(5):442-51. doi: 10.2353/jmoldx.2008.080033. Epub 2008 
      Aug 7.