PMID- 18690394
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 60
IP  - 4
DP  - 2008 Aug 25
TI  - [NADPH oxidase activity does not affect cellular cholesterol loading in vascular 
      smooth muscle cells].
PG  - 511-9
AB  - Reactive oxygen species generated by NADPH oxidase enhance aortic vascular smooth 
      muscle cell proliferation and migration which play an important role in the 
      pathophysiology of atherosclerosis. We investigated the role of NADPH oxidase in 
      the cellular cholesterol metabolism in vascular smooth muscle cells using 
      p47phox-deficient cells. Wild-type and p47phox knockout vascular smooth muscle 
      cells were loaded with cholesterol for 72 h by using 10 mg/L 
      cholesterol:methyl-beta-cyclodextrin complexes and then incubated with or without 
      0.3 mg/L thrombin for 10 min. Foam cell formation was determined by accumulation 
      of intracellular cholesterol, oil Red O-stained lipid droplets. After cholesterol 
      loading, cellular lipid droplets raised sharply, cellular cholesterol increased 
      from (31.4+/-2.0) to (61.0+/-2.1) mg/g protein (P<0.05) in wild-type cells, and 
      from (29.8+/-2.5) to (51.3+/-3.1) mg/g protein (P<0.05) in p47phox deficient 
      cells, but the difference between the two cell types was not significant. 
      Immunostaining showed decreased levels of smooth muscle alpha-actin and increased 
      levels of macrophage marker Mac-2 in both wild-type and p47phox deficient 
      vascular smooth muscle cells. One of the macrophage-related inflammation genes, 
      monocyte chemoattractant protein-1 (MCP-1) expression did not change in both two 
      cell types detected by immunostaining. Although additional incubating with 
      thrombin, another macrophage-related inflammation gene, vascular cell adhesion 
      molecule-1 (VCAM-1) expression was similar in all groups analyzed by real-time 
      RT-PCR. However, the expression of ATP-binding cassette transporter A1 (ABCA1), 
      acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1), the key proteins in 
      cellular cholesterol metabolism, were similarly increased (P<0.05) in both two 
      cell types as determined by quantitative real-time RT-PCR and Western blot, and 
      it was not related to the state of oxidative stress. Interestingly, the 
      expression of adipophilin, the lipid droplet related protein, had the similar 
      results with ABCA1 and ACAT1, but, in wild-type cells, its expression also 
      increased merely incubating with thrombin as determined by quantitative real-time 
      RT-PCR. Together, these results suggest that p47phox-dependent NADPH oxidase is 
      not involved in transdifferentitation of vascular smooth muscle cells into 
      macrophage-like state after cholesterol loading. Deleting p47phox gene does not 
      affect the cellular cholesterol metabolism in vascular smooth muscle cells.
FAU - Yuan, Zhong-Hua
AU  - Yuan ZH
AD  - Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of 
      Hunan Province, University of South China, Hengyang 421001, China.
FAU - Madamanchi, Nageswara R
AU  - Madamanchi NR
FAU - Vendrov, Aleksandr E
AU  - Vendrov AE
FAU - Niu, Xi-Lin
AU  - Niu XL
FAU - Li, Ju-Xiang
AU  - Li JX
FAU - Runge, Marschall S
AU  - Runge MS
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 2.3.1.26 (Sterol O-Acyltransferase)
RN  - EC 2.3.1.26 (sterol O-acyltransferase 1)
SB  - IM
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Cholesterol/*metabolism
MH  - Foam Cells/cytology
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/*enzymology
MH  - NADPH Oxidases/*metabolism
MH  - RNA, Messenger
MH  - Sterol O-Acyltransferase/metabolism
MH  - beta-Cyclodextrins/pharmacology
EDAT- 2008/08/12 09:00
MHDA- 2015/05/20 06:00
CRDT- 2008/08/12 09:00
PHST- 2008/08/12 09:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PHST- 2008/08/12 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2008 Aug 25;60(4):511-9.