PMID- 18692128 OWN - NLM STAT- MEDLINE DCOM- 20090206 LR - 20081125 IS - 0891-0618 (Print) IS - 0891-0618 (Linking) VI - 36 IP - 3-4 DP - 2008 Dec TI - Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys. PG - 138-43 LID - 10.1016/j.jchemneu.2008.07.004 [doi] AB - Neurotrophins (NTs) appear to be crucial for the survival and potential regeneration of injured neurons. However, their temporal changes and remote regulations following spinal cord injury (SCI) have been only partially determined, especially in primates. In this study, ELISA was performed on the extracts of injured spinal cord and the associated precentral gyrus contralateral to the site of spinal cord hemisection to investigate the temporal changes in the levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) in adult rhesus monkeys subjected to T8 spinal hemisection. Animals were allowed to survive 3, 7, 14, 30 and 90 days post-operation (dpo). In the spinal cord, the levels of NGF, BDNF and NT-3 sharply decreased between 3 and 7dpo. Thereafter, the levels of NGF and BDNF were transiently elevated while NT-3 level continuously increased and recovered to normal level at 30dpo. In the contralateral precentral gyrus (cPG), only the NT-3 level was altered and in fact elevated above the normal value. No obvious changes were observed in NT-4 level in any of the regions studied. Taken together, the present findings indicated that intrinsic NGF, BDNF and NT-3 may play a local role in the responses to the SCI in primates. Especially, the increase of NT-3 level occurred continuously in both the cPG and the spinal cord pointed to a possible transportation of NT-3 to the cord following SCI. FAU - Zhang, Hong-Tian AU - Zhang HT AD - Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Gao, Zhi-Yu AU - Gao ZY FAU - Chen, Yi-Zhao AU - Chen YZ FAU - Wang, Ting-Hua AU - Wang TH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080722 PL - Netherlands TA - J Chem Neuroanat JT - Journal of chemical neuroanatomy JID - 8902615 RN - 0 (Actins) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 39379-15-2 (Neurotensin) SB - IM MH - Actins/metabolism MH - Animals MH - Blotting, Western MH - Brain-Derived Neurotrophic Factor/metabolism MH - Enzyme-Linked Immunosorbent Assay MH - Immunohistochemistry MH - Macaca mulatta MH - Male MH - Nerve Growth Factors/*metabolism MH - Neurotensin/metabolism MH - Spinal Cord/*metabolism EDAT- 2008/08/12 09:00 MHDA- 2009/02/07 09:00 CRDT- 2008/08/12 09:00 PHST- 2008/02/05 00:00 [received] PHST- 2008/07/10 00:00 [revised] PHST- 2008/07/10 00:00 [accepted] PHST- 2008/08/12 09:00 [pubmed] PHST- 2009/02/07 09:00 [medline] PHST- 2008/08/12 09:00 [entrez] AID - S0891-0618(08)00093-8 [pii] AID - 10.1016/j.jchemneu.2008.07.004 [doi] PST - ppublish SO - J Chem Neuroanat. 2008 Dec;36(3-4):138-43. doi: 10.1016/j.jchemneu.2008.07.004. Epub 2008 Jul 22.