PMID- 18692563
OWN - NLM
STAT- MEDLINE
DCOM- 20081208
LR  - 20181201
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 26
IP  - 2
DP  - 2008 Oct
TI  - Action of defined mixtures of PCBs, p,p'-DDT and its metabolite p,p'-DDE, on 
      co-culture of porcine theca and granulosa cells: steroid secretion, cell 
      proliferation and apoptosis.
PG  - 170-4
LID - 10.1016/j.reprotox.2008.07.003 [doi]
AB  - We recently reported on a stimulatory effect of a mixture of persistent organic 
      pollutants (POPs) extracted from the liver oil of Atlantic cod (Gadus morhua) on 
      estradiol (E2) secretion by porcine ovarian follicular cells [Gregoraszczuk EL, 
      Milczarek K, Wojtowicz A, Berg V, Skaare JU, Ropstad E. Steroid secretion 
      following exposure of ovarian follicular cells to three different natural 
      mixtures of persistent organic pollutants (POPs). Reproductive Toxicol 
      2007;25:58-66]. The objective of the present study was to identify what compound 
      or compounds within this natural mixture could be responsible for the stimulatory 
      action on E2 secretion. Co-cultures of porcine ovarian theca and granulosa cells 
      were exposed for 48h to individual congeners of polychlorinated biphenyls (PCBs) 
      alone or in combination, at environmentally relevant concentrations (PCB153 at 
      8microg/ml; PCB118 at 3microg/ml; PCB138 at 8microg/ml; PCB180 at 3microg/ml; 
      PCB126 at 8ng/ml; DDT at 5microg/ml; p,p'-DDE at 10microg/ml). At the end of 
      culture media samples were collected and used for cell viability and steroid 
      determination, and cells were used for the measurement of caspase-3 activity. A 
      threefold decrease in E2 secretion was noted when PCB126 was added in combination 
      with PCB153. Only DDT and DDE could reverse the action of PCB126. Stimulatory 
      secretion of E2 occurred in parallel with a decrease in testosterone (T) 
      secretion. Among the single congeners used in the present study, DDE had the 
      strongest stimulatory action on E2 secretion. In conclusion, the present findings 
      indicate that the overall effect on steroid secretion by ovarian follicular cells 
      could be dependent on the concentration of DDE in environmental POPs and that the 
      predicted effect of a mixture based on a sum of independent effects of individual 
      congeners is higher than that noted under the influence of defined mixture.
FAU - Gregoraszczuk, Ewa L
AU  - Gregoraszczuk EL
AD  - Department of Physiology and Toxicology of Reproduction, Chair of Animal 
      Physiology, Institute of Zoology, Jagiellonian University, Krakow, Poland. 
      ewa.gregoraszczuk@uj.edu.pl
FAU - Ptak, Anna
AU  - Ptak A
FAU - Karniewska, Magdalena
AU  - Karniewska M
FAU - Ropstad, Erik
AU  - Ropstad E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080722
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Environmental Pollutants)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 3XMK78S47O (Testosterone)
RN  - 4M7FS82U08 (Dichlorodiphenyl Dichloroethylene)
RN  - 4TI98Z838E (Estradiol)
RN  - CIW5S16655 (DDT)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - DDT/chemistry/metabolism/toxicity
MH  - Dichlorodiphenyl Dichloroethylene/chemistry/metabolism/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Environmental Pollutants/chemistry/metabolism/*toxicity
MH  - Estradiol/metabolism
MH  - Female
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Granulosa Cells/cytology/*drug effects/metabolism
MH  - Polychlorinated Biphenyls/chemistry/metabolism/toxicity
MH  - Swine
MH  - Testosterone/metabolism
MH  - Theca Cells/cytology/*drug effects/metabolism
EDAT- 2008/08/12 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/12 09:00
PHST- 2007/11/06 00:00 [received]
PHST- 2008/06/11 00:00 [revised]
PHST- 2008/07/10 00:00 [accepted]
PHST- 2008/08/12 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/12 09:00 [entrez]
AID - S0890-6238(08)00172-X [pii]
AID - 10.1016/j.reprotox.2008.07.003 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2008 Oct;26(2):170-4. doi: 10.1016/j.reprotox.2008.07.003. Epub 
      2008 Jul 22.