PMID- 18696220
OWN - NLM
STAT- MEDLINE
DCOM- 20090604
LR  - 20231213
IS  - 1573-2592 (Electronic)
IS  - 0271-9142 (Linking)
VI  - 29
IP  - 1
DP  - 2009 Jan
TI  - In vitro differentiation of human monocytes into dendritic cells by 
      peptic-tryptic digest of gliadin is independent of genetic predisposition and the 
      presence of celiac disease.
PG  - 29-37
LID - 10.1007/s10875-008-9228-x [doi]
AB  - INTRODUCTION: This study was done to further reveal the role of the innate immune 
      system in celiac disease. METHODS: Dendritic cells were matured from venous blood 
      of patients with active or treated celiac disease and DQ2-DQ8-positive or 
      negative controls. Dendritic cells were treated with a peptic-tryptic digest of 
      gliadin (500 microg/ml) and their activation was analyzed by 
      fluorescent-activated cell sorting analysis, cytokine secretion, and their 
      ability to elicit T cell proliferation. RESULTS AND DISCUSSION: Gliadin 
      upregulated interleukin (IL)-6, IL-8, and IL-12 (p40) secretion in dendritic 
      cells and induced strong expression of the maturation markers human leukocyte 
      antigen (HLA)-DR, CD25, CD83, and CD86 of all subjects irrespective of their 
      genotype or the presence of disease, whereas the digest of bovine serum albumin 
      showed no effect. However, gliadin-stimulated dendritic cells from active celiac 
      showed enhanced stimulation of autologous T cells compared to the other groups. 
      CONCLUSION: Further research should be aimed at identifying the mechanisms that 
      control inflammation in healthy individuals.
FAU - Rakhimova, Maryam
AU  - Rakhimova M
AD  - Department of Medicine 1, University Erlangen-Nuernberg, 91054, Erlangen, 
      Germany.
FAU - Esslinger, Birgit
AU  - Esslinger B
FAU - Schulze-Krebs, Anja
AU  - Schulze-Krebs A
FAU - Hahn, Eckhart G
AU  - Hahn EG
FAU - Schuppan, Detlef
AU  - Schuppan D
FAU - Dieterich, Walburga
AU  - Dieterich W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080812
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Membrane Glycoproteins)
RN  - 9007-90-3 (Gliadin)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/immunology/metabolism
MH  - B7-2 Antigen/immunology/metabolism
MH  - Celiac Disease/genetics/*immunology/metabolism
MH  - Cytokines/biosynthesis/immunology
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Gliadin/chemistry/*immunology/pharmacology
MH  - HLA-DR Antigens/immunology/metabolism
MH  - Humans
MH  - Immunoglobulins/immunology/metabolism
MH  - Interleukin-2 Receptor alpha Subunit/immunology/metabolism
MH  - Male
MH  - Membrane Glycoproteins/immunology/metabolism
MH  - Middle Aged
MH  - Monocytes/drug effects/*immunology/metabolism
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Trypsin/chemistry
MH  - Up-Regulation/immunology
MH  - CD83 Antigen
EDAT- 2008/08/13 09:00
MHDA- 2009/06/06 09:00
CRDT- 2008/08/13 09:00
PHST- 2008/06/24 00:00 [received]
PHST- 2008/07/21 00:00 [accepted]
PHST- 2008/08/13 09:00 [pubmed]
PHST- 2009/06/06 09:00 [medline]
PHST- 2008/08/13 09:00 [entrez]
AID - 10.1007/s10875-008-9228-x [doi]
PST - ppublish
SO  - J Clin Immunol. 2009 Jan;29(1):29-37. doi: 10.1007/s10875-008-9228-x. Epub 2008 
      Aug 12.