PMID- 18701419
OWN - NLM
STAT- MEDLINE
DCOM- 20080918
LR  - 20191210
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 130
IP  - 3
DP  - 2008 Sep
TI  - Prothrombinase-induced clotting time assay for determination of the anticoagulant 
      effects of unfractionated and low-molecular-weight heparins, fondaparinux, and 
      thrombin inhibitors.
PG  - 446-54
LID - 10.1309/Q0G21Y26UR0UHQ1A [doi]
AB  - The prothrombinase-induced clotting time assay (PiCT, Pentapharm, Basel, 
      Switzerland) is a clotting assay sensitive to factor Xa and factor IIa 
      inhibitors. It is based on the addition of factor Xa and snake venom RVV-V 
      (Russell viper venom factor V activator) specifically activating factor V and 
      phospholipids to platelet-poor plasma. Following an incubation time, the mixture 
      is recalcified and the clotting time is determined. An almost linear 
      dose-response and high sensitivity of the assay for unfractionated heparin (UFH), 
      low-molecular-weight heparins (LMWHs), r-hirudin, and argatroban was found. 
      Fondaparinux showed a nonlinear dose-response. By using ex vivo samples, the 
      following Pearson correlation coefficients were found: r=0.85 between amidolytic 
      anti-Xa and PiCT for 120 LMWH and 24 control samples; r=0.86 between amidolytic 
      anti-Xa activity and PiCT for 68 UFH and 24 control samples; and r=0.94 between 
      ECT and PiCT for 38 hirudin samples. Thus, PiCT is a promising assay for the 
      monitoring of anticoagulants inhibiting factor Xa and/or factor IIa.
FAU - Calatzis, Andreas
AU  - Calatzis A
AD  - Department of Haemostasis and Transfusion Medicine, Munich University Clinic, 
      Munich, Germany.
FAU - Peetz, Dirk
AU  - Peetz D
FAU - Haas, Sylvia
AU  - Haas S
FAU - Spannagl, Michael
AU  - Spannagl M
FAU - Rudin, Karin
AU  - Rudin K
FAU - Wilmer, Marianne
AU  - Wilmer M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Polysaccharides)
RN  - 9005-49-6 (Heparin)
RN  - 9035-58-9 (Thromboplastin)
RN  - J177FOW5JL (Fondaparinux)
SB  - IM
MH  - Blood Coagulation Tests/*methods
MH  - Fibrinolytic Agents/*therapeutic use
MH  - Fondaparinux
MH  - Heparin/*therapeutic use
MH  - Heparin, Low-Molecular-Weight/*therapeutic use
MH  - Humans
MH  - Polysaccharides/*therapeutic use
MH  - Sensitivity and Specificity
MH  - Thromboplastin/*physiology
EDAT- 2008/08/15 09:00
MHDA- 2008/09/19 09:00
CRDT- 2008/08/15 09:00
PHST- 2008/08/15 09:00 [pubmed]
PHST- 2008/09/19 09:00 [medline]
PHST- 2008/08/15 09:00 [entrez]
AID - 1114U6054XR26403 [pii]
AID - 10.1309/Q0G21Y26UR0UHQ1A [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2008 Sep;130(3):446-54. doi: 10.1309/Q0G21Y26UR0UHQ1A.