PMID- 18704330
OWN - NLM
STAT- MEDLINE
DCOM- 20080916
LR  - 20131121
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 31
IP  - 7
DP  - 2008 Jul
TI  - HPLC determination of malondialdehyde in ECV304 cell culture medium for measuring 
      the antioxidant effect of vitexin-4''-O-glucoside.
PG  - 878-85
LID - 10.1007/s12272-001-1241-2 [doi]
AB  - To investigate the antioxidant effect of vitexin-4''-O-glucoside, a flavone 
      glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we 
      developed a simple and sensitive high-performance liquid chromatography (HPLC) 
      method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium 
      after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed 
      samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC 
      analysis was performed on a Synergi Hydro-RP, a polar end-capped C18 column (250 
      x 4.6 mm, 4 mum), using an acetonitrile-ammonium acetate aqueous solution (10 mM, 
      pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 
      532 nm. The calibration curve was linear over 0.0125-1.25 microM MDA (r = 
      0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision 
      were less than 6.1% and 5.0%, respectively. The mean recovery was 96.9 +/- 1.6%. 
      The lower limit of quantification (LLOQ) of MDA was 0.0125 microM. This 
      chromatographic method was successfully applied to investigating the in vitro 
      antioxidant effect of vitexin-4''-O-glucoside. Vitexin-4''-O-glucoside (120 M) 
      protected ECV304 cells from peroxidation induced by TBHP.
FAU - Ying, Xi-xiang
AU  - Ying XX
AD  - School of Pharmacy, Liaoning University of TCM, No. 79 Chongshan East Road, 
      Shenyang, China.
FAU - Li, Hai-bo
AU  - Li HB
FAU - Chu, Zheng-yun
AU  - Chu ZY
FAU - Zhai, Yan-jun
AU  - Zhai YJ
FAU - Leng, Ai-jing
AU  - Leng AJ
FAU - Liu, Xun
AU  - Liu X
FAU - Xin, Chun
AU  - Xin C
FAU - Zhang, Wen-jie
AU  - Zhang WJ
FAU - Kang, Ting-guo
AU  - Kang TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080814
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
RN  - 0 (Antioxidants)
RN  - 0 (Culture Media)
RN  - 0 (Glucosides)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Isoflavones)
RN  - 0 (Oxidants)
RN  - 0 (isovitexin-4'-O-glucoside)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 955VYL842B (tert-Butylhydroperoxide)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Crataegus/chemistry
MH  - Culture Media/analysis
MH  - Glucosides/*pharmacology
MH  - Indicators and Reagents
MH  - Isoflavones/*pharmacology
MH  - Magnetic Resonance Spectroscopy
MH  - Malondialdehyde/*analysis/*metabolism
MH  - Oxidants/toxicity
MH  - Plant Leaves/chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Spectrophotometry, Ultraviolet
MH  - tert-Butylhydroperoxide/toxicity
EDAT- 2008/08/16 09:00
MHDA- 2008/09/17 09:00
CRDT- 2008/08/16 09:00
PHST- 2008/01/14 00:00 [received]
PHST- 2008/06/11 00:00 [accepted]
PHST- 2008/03/27 00:00 [revised]
PHST- 2008/08/16 09:00 [pubmed]
PHST- 2008/09/17 09:00 [medline]
PHST- 2008/08/16 09:00 [entrez]
AID - 10.1007/s12272-001-1241-2 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2008 Jul;31(7):878-85. doi: 10.1007/s12272-001-1241-2. Epub 2008 
      Aug 14.