PMID- 18705004
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20220327
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 37
IP  - 4
DP  - 2008 Jul
TI  - [Effect of Cryptoporus polysaccharide on lipopolysaccharide-induced production of 
      monocyte chemoattractant protein-1 in human alveolar epithelial cells].
PG  - 333-9
AB  - OBJECTIVE: To investigate the effect of cryptoporus polysaccharide(CP)on 
      lipopolysaccharide(LPS)-induced production of monocyte chemoattractant 
      protein-1(MCP-1)in human lung epithelial A549 cells. METHODS: A549 cells were 
      stimulated with LPS in the presence or absence of CP. The protein concentration 
      and mRNA expression of MCP-1 were determined by enzyme-linked-immunosobent 
      assay(ELISA)and semi-quantitative RT-PCR, respectively. RESULT: The protein 
      concentration of MCP-1 was significantly increased by LPS 1000 microg/L at 24 h. 
      There were no effects on the growth and viability of A549 cells in the presence 
      of CP 100 microg/L or dexamethasone 1 mumol/L. However, CP 100 microg/L or 
      dexamethasone 1 micromol/L significantly inhibited the protein concentration and 
      mRNA expression of MCP-1 induced by LPS. CONCLUSION: CP can regulate MCP-1 
      production, which may be associated with its effects on lung inflammation.
FAU - Zhu, Jianing
AU  - Zhu J
AD  - Pharmacy of Affiliated Sir Run Run Shaw Hospital of Zhejiang University Medical 
      College, Hangzhou 310016, China.
FAU - Zhang, Hui
AU  - Zhang H
FAU - Xie, Qiangmin
AU  - Xie Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Epithelial Cells/cytology/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Polyporaceae/*chemistry
MH  - Polysaccharides/isolation & purification/*pharmacology
MH  - Pulmonary Alveoli/*cytology
MH  - RNA, Messenger/metabolism
EDAT- 2008/08/16 09:00
MHDA- 2010/03/31 06:00
CRDT- 2008/08/16 09:00
PHST- 2008/08/16 09:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
PHST- 2008/08/16 09:00 [entrez]
AID - 10.3785/j.issn.1008-9292.2008.04.003 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2008 Jul;37(4):333-9. doi: 
      10.3785/j.issn.1008-9292.2008.04.003.