PMID- 18705801
OWN - NLM
STAT- MEDLINE
DCOM- 20081110
LR  - 20211020
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 116
IP  - 4
DP  - 2008 Aug
TI  - Expression of endothelial monocyte-activating polypeptide II in the rat dental 
      follicle and its potential role in tooth eruption.
PG  - 334-40
LID - 10.1111/j.1600-0722.2008.00547.x [doi]
AB  - Endothelial monocyte-activating polypeptide II (EMAP-II) is an inflammatory 
      cytokine with chemotactic activity. Because the dental follicle (DF) recruits 
      mononuclear cells (osteoclast precursors) to promote the osteoclastogenesis 
      needed for tooth eruption, it was the aim of this study to determine if EMAP-II 
      contributes to this recruitment. Using a DNA microarray, EMAP-II was found to be 
      highly expressed in vivo in the DFs of day 1 to day 11 postnatal rats, with its 
      expression elevated on days 1 and 3. Use of a short interfering RNA (siRNA) to 
      knock down EMAP-II expression resulted in a reduction in the expression of 
      colony-stimulating factor-1 (CSF-1) and monocyte chemoattractant protein-1 
      (MCP-1) in the DF cells. Addition of EMAP-II protein to the DF cells partially 
      restored the expression of CSF-1 and MCP-1. In chemotaxis assays using either 
      conditioned medium of the DF cells with anti-(EMAP-II) immunoglobulin G added or 
      conditioned medium of DF cells with EMAP-II knocked down by siRNA, migration 
      indexes of bone marrow mononuclear cells were significantly reduced. These 
      results suggest that EMAP-II is another chemotactic molecule in the dental 
      follicle involved in the recruitment of mononuclear cells, and that EMAP-II may 
      exert its chemotactic function directly by recruiting mononuclear cells and 
      indirectly by enhancing the expression of other chemotactic molecules (CSF-1 and 
      MCP-1).
FAU - Liu, Dawen
AU  - Liu D
AD  - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, LA 70803, USA.
FAU - Wise, Gary E
AU  - Wise GE
LA  - eng
GR  - R01 DE008911/DE/NIDCR NIH HHS/United States
GR  - R01 DE008911-16/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (small inducible cytokine subfamily E, member 1)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemotaxis, Leukocyte
MH  - Cytokines/*biosynthesis/*physiology
MH  - Dental Sac/*metabolism
MH  - Leukocytes, Mononuclear/physiology
MH  - Macrophage Colony-Stimulating Factor/biosynthesis
MH  - Neoplasm Proteins/*biosynthesis/*physiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Osteoclasts/physiology
MH  - RNA Interference
MH  - RNA-Binding Proteins/*biosynthesis/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tooth Eruption/*physiology
PMC - PMC2597391
MID - NIHMS65027
EDAT- 2008/08/19 09:00
MHDA- 2008/11/11 09:00
PMCR- 2009/08/01
CRDT- 2008/08/19 09:00
PHST- 2008/08/19 09:00 [pubmed]
PHST- 2008/11/11 09:00 [medline]
PHST- 2008/08/19 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - EOS547 [pii]
AID - 10.1111/j.1600-0722.2008.00547.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2008 Aug;116(4):334-40. doi: 10.1111/j.1600-0722.2008.00547.x.