PMID- 18705843
OWN - NLM
STAT- MEDLINE
DCOM- 20081009
LR  - 20080818
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 60
IP  - 2
DP  - 2008 Aug
TI  - Repertoire and clonality of T-cell receptor beta variable genes expressed in 
      endometrium and blood T cells of patients with recurrent spontaneous abortion.
PG  - 160-71
LID - 10.1111/j.1600-0897.2008.00608.x [doi]
AB  - PROBLEM: Recurrent spontaneous abortion (RSA) is a relatively common disorder, 
      the underlying causes of which are thought to be immunological in most cases. 
      METHOD OF STUDY: Expression profile and clonality pattern of T-cell receptor beta 
      variable (TCRBV) genes in endometrium and blood of patients with RSA were 
      investigated by semi-quantitative reverse transcriptase polymerase chain reaction 
      (RT-PCR) using BV gene-specific primers. Relative expression of each BV family 
      was determined and clonal expansion of the over-expressed genes was assessed by 
      analysis of CDR3 length polymorphism. RESULTS: Compared to blood, relative 
      expression of four TCRBV genes was significantly higher in the endometrium of RSA 
      group. Over-expressed genes, except for TCRBV3, all had restricted and 
      oligoclonal patterns of expression in the endometrium. CONCLUSION: Endometrial T 
      cells have a skewed TCRBV repertoire with restricted transcript heterogeneity, 
      which is shared by both groups and minor variations observed in this pattern in 
      RSA patients may reflect more recent and/or repeated exposure to nominal antigens 
      or superantigens.
FAU - Dokouhaki, Pouneh
AU  - Dokouhaki P
AD  - Department of Immunology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran. pouneh_d@yahoo.com
FAU - Moghaddam, Rosa
AU  - Moghaddam R
FAU - Rezvany, MohammadReza
AU  - Rezvany M
FAU - Ghassemi, Jamileh
AU  - Ghassemi J
FAU - Novin, Marefat Ghaffari
AU  - Novin MG
FAU - Zarnani, Amirhassan
AU  - Zarnani A
FAU - Akhondi, Mohammah-Mehdi
AU  - Akhondi MM
FAU - Ostadkarampour, Mahyar
AU  - Ostadkarampour M
FAU - Mellstedt, Hakan
AU  - Mellstedt H
FAU - Razavi, Alireza
AU  - Razavi A
FAU - Jeddi-Tehrani, Mahmood
AU  - Jeddi-Tehrani M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Abortion, Habitual/genetics/*immunology/metabolism
MH  - Adult
MH  - Case-Control Studies
MH  - Complementarity Determining Regions/genetics
MH  - Endometrium/*immunology/metabolism
MH  - Female
MH  - Gene Expression
MH  - *Genes, T-Cell Receptor beta
MH  - Humans
MH  - Polymorphism, Genetic
MH  - Pregnancy
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics/immunology/*metabolism
MH  - T-Lymphocytes/*immunology/metabolism
EDAT- 2008/08/19 09:00
MHDA- 2008/10/10 09:00
CRDT- 2008/08/19 09:00
PHST- 2008/08/19 09:00 [pubmed]
PHST- 2008/10/10 09:00 [medline]
PHST- 2008/08/19 09:00 [entrez]
AID - AJI608 [pii]
AID - 10.1111/j.1600-0897.2008.00608.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2008 Aug;60(2):160-71. doi: 
      10.1111/j.1600-0897.2008.00608.x.