PMID- 18706448
OWN - NLM
STAT- MEDLINE
DCOM- 20090205
LR  - 20191210
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 154
IP  - 1-2
DP  - 2008 Dec
TI  - Loop-mediated isothermal amplification for discriminating between human 
      herpesvirus 6 A and B.
PG  - 223-5
LID - 10.1016/j.jviromet.2008.07.004 [doi]
AB  - Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly 
      for the diagnosis of neurological diseases. The objective of this study was to 
      establish a rapid HHV-6 genotyping method using the loop-mediated isothermal 
      amplification (LAMP) method. An AccI site is located in the target sequence of 
      HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and 
      then separated by agarose gel electrophoresis to differentiate the digest pattern 
      of the two variants. The fragment patterns were clearly different between HHV-6 A 
      and B. In order to evaluate the reliability of this HHV-6 genotyping method for 
      use in the clinical laboratory, serum samples from 20 patients with either 
      primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 
      DNA was amplified directly from the serum samples and all 20 LAMP products were 
      positive for HHV-6 B.
FAU - Ihira, Masaru
AU  - Ihira M
AD  - Department of Medical Information Technology, Fujita Health University College, 
      Toyoake, Aichi, Japan.
FAU - Ohta, Akane
AU  - Ohta A
FAU - Sugata, Ken
AU  - Sugata K
FAU - Suga, Sadao
AU  - Suga S
FAU - Asano, Yoshizo
AU  - Asano Y
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20081011
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GTMKAC-specific type II deoxyribonucleases)
SB  - IM
MH  - DNA, Viral/*genetics/metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Electrophoresis, Agar Gel
MH  - Genotype
MH  - Herpesvirus 6, Human/*classification/*isolation & purification
MH  - Humans
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - *Polymorphism, Restriction Fragment Length
EDAT- 2008/08/19 09:00
MHDA- 2009/02/06 09:00
CRDT- 2008/08/19 09:00
PHST- 2008/04/19 00:00 [received]
PHST- 2008/06/30 00:00 [revised]
PHST- 2008/07/17 00:00 [accepted]
PHST- 2008/08/19 09:00 [pubmed]
PHST- 2009/02/06 09:00 [medline]
PHST- 2008/08/19 09:00 [entrez]
AID - S0166-0934(08)00248-6 [pii]
AID - 10.1016/j.jviromet.2008.07.004 [doi]
PST - ppublish
SO  - J Virol Methods. 2008 Dec;154(1-2):223-5. doi: 10.1016/j.jviromet.2008.07.004. 
      Epub 2008 Oct 11.