PMID- 18706910
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20211028
IS  - 1089-8638 (Electronic)
IS  - 0022-2836 (Linking)
VI  - 382
IP  - 5
DP  - 2008 Oct 24
TI  - The malignant brain tumor repeats of human SCML2 bind to peptides containing 
      monomethylated lysine.
PG  - 1107-12
LID - 10.1016/j.jmb.2008.07.081 [doi]
AB  - SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive 
      complex 1, a large multiprotein assembly required for the repression of 
      developmental control genes. It contains two MBT (malignant brain tumor) repeats; 
      the MBT is a protein module structurally similar to domains that bind to 
      methylated histones. We have used NMR spectroscopy to examine the binding 
      specificity of these repeats. Our data show that they preferentially bind histone 
      peptides monomethylated at lysine residues with no apparent sequence specificity. 
      The crystal structure of the complex between the protein and monomethyllysine 
      reveals that the modified amino acid binds to an aromatic rich pocket at one end 
      of the beta-barrel of the second repeat.
FAU - Santiveri, Clara M
AU  - Santiveri CM
AD  - MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 2QH, UK.
FAU - Lechtenberg, Bernhard C
AU  - Lechtenberg BC
FAU - Allen, Mark D
AU  - Allen MD
FAU - Sathyamurthy, Aruna
AU  - Sathyamurthy A
FAU - Jaulent, Agnes M
AU  - Jaulent AM
FAU - Freund, Stefan M V
AU  - Freund SM
FAU - Bycroft, Mark
AU  - Bycroft M
LA  - eng
SI  - PDB/2VYT
GR  - MC_U105459896/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080803
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
RN  - 0 (Polycomb-Group Proteins)
RN  - 0 (SCML2 protein, human)
RN  - 0 (Transcription Factors)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Binding Sites
MH  - DNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Epigenesis, Genetic
MH  - Histones/*chemistry/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Ligands
MH  - Lysine/analogs & derivatives/chemistry
MH  - Methylation
MH  - Models, Molecular
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Peptides/chemistry/metabolism
MH  - Polycomb-Group Proteins
MH  - Protein Binding
MH  - Protein Conformation
MH  - Repetitive Sequences, Amino Acid
MH  - Transcription Factors/*chemistry/genetics/*metabolism
EDAT- 2008/08/19 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/08/19 09:00
PHST- 2008/06/18 00:00 [received]
PHST- 2008/07/21 00:00 [accepted]
PHST- 2008/08/19 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/08/19 09:00 [entrez]
AID - S0022-2836(08)00950-9 [pii]
AID - 10.1016/j.jmb.2008.07.081 [doi]
PST - ppublish
SO  - J Mol Biol. 2008 Oct 24;382(5):1107-12. doi: 10.1016/j.jmb.2008.07.081. Epub 2008 
      Aug 3.